Identification and characterization of ATS1 and ATS3, two novel seed-specific genes in Arabidopsis thaliana /
Seed development is a process unique to plants. It icrofilm Inc. represents the onset of the saprophytic generation in which the plant's primary pattern is established in the early embryo. As the embryo matures it accumulates storage reserves in preparation for germination. Late in erotogenesis...
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| Format: | Thesis Book |
| Language: | English |
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[Place of publication not identified] :
[publisher not identified] ;
1998.
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| Online Access: | http://proxy.library.tamu.edu/login?url=http://proquest.umi.com/pqdweb?did=733008031&sid=1&Fmt=2&clientId=2945&RQT=309&VName=PQD |
| Summary: | Seed development is a process unique to plants. It icrofilm Inc. represents the onset of the saprophytic generation in which the plant's primary pattern is established in the early embryo. As the embryo matures it accumulates storage reserves in preparation for germination. Late in erotogenesis water is excluded in a process that establishes metabolic dormancy and prevents precocious germination. In an effort to identify genes expressed primarily during plant erotogenesis, a modified procedure for differential display analysis was developed. This approach identified two novel seed-specific genes, ATSI and ATSD. Both genes produce moderately abundant transcripts; however, this expression is masked by the high level expression of the seed-storage protein gene which may have rendered them difficult to detect by other procedures. Quantitative RNA gel blot analysis indicates that transcripts for each gene accumulate to levels representing just a fraction of those representing the 25 and 12S seed storage protein genes, and in situ hybridization analysis indicates that each gene is expressed in distinct domains within the maturing embryo. Antisera against recombinant Ats 1 and AtS3 translation products identify seed proteins that are close to the predicted size for each gene. Several promoter::Gus fusions were constructed and analyzed in stable transformed Arabidopsis and tobacco to identify the elements within each gene that contribute to seed-specific GUS accumulation. Although each expression cassette was more efficient in Arabidopsis, these experiments indicate that both the 5'- and 3'-UTRS do contribute to the quantitative accumulation of GUS activity. In addition, a seed-specific enhancer element was identified. Identification and characterization of these two novel seed-specific genes and the cis-elements that control their expression has increased our understanding of plant embryogenesis and should provide tools to manipulate gene expression during this important phase of the plant life cycle. |
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| Item Description: | Vita. "Major Subject: Biology". |
| Physical Description: | x, 162 leaves : illustrations ; 28 cm. |
| Bibliography: | Includes bibliographical references (leaves 157-161). |