Use of heparin to accelerate capacitation of equine spermatozoa in vivo /

10 gg/ml). Timing of postovulation insemination tended

Bibliographic Details
Main Author: Fleet Nelson, Tami Lynn , 1967-
Format: Thesis eBook
Language:English
Published: [Place of publication not identified] : [publisher not identified] ; 1998.
Subjects:
Online Access:Link to OAKTrust copy
Description
Summary:10 gg/ml). Timing of postovulation insemination tended
7;43%). For mares bred at 12-24 h after ovulation, a
accelerates capacitation of spermatozoa under in vivo
all data, insemination with gelatinized semen
capacitation of equine spermatozoa in vivo. The
capacitation of stallion spermatozoa in vivo, one
conditions, thereby enhancing fertilization of locates
detection of ovulation. This experiment was designed
experimental protocol involved insemination of mares
extender would enhance pregnancy rates when
following insemination with nonheparirlized semen (5
gelatinized semen yielded a higher (p = 0.003)
in the early postovulatory period. Oocyte viability is
increased (p = 0.01) the pregnancy rate in mares (12
inseminated either 0-12 h or 12-24 h after ovulation,
insemination with nonheparinized semen (3 out of
insemination/breeding is conducted only after
inseminations were performed following ovulation. To
investigate the ability of heparin to accelerate
is performed following ovulation.
known to be relatively short following ovulation,
Nonetheless, these data suggest that heparin
of accelerating capacitation of equine spermatozoa
out of 16,. 31%). The effect of heparin on spermatozoa
out of 16,* 75%), as compared to pregnancy rate
pregnancy rate (7 out of 7,. 100%) than did
Previous studies have revealed that heparin is capable ics.
prior to loss of oocyte viability, when insemination
should be tested on additional stallions to determine
spermatozoa pre-incubated in heparin containing
stallion and 32 mares were utilized. Mares were
thereby leading to lowered pregnancy rates when
to affect pregnancy rate (p = 0.07), with an advantage
to determine whether insemination of mares with
to earlier postovulation breeding. For mares bred
treatment effect was not detected (p = 0. 17). Over
under in vitro conditions. The objective of this study
was to evaluate the ability of heparin to accelerate
whether individual stallion variation exists.
with or without added heparin (final concentration of
with semen diluted in a nonfat milk-glucose extender,
within 12 h after ovulation, insemination with
Item Description:"Major subject: Physiology of Reproduction".
Vita.
Physical Description:x, 63 leaves : illustrations ; 28 cm.
Also available online.
Bibliography:Includes bibliographical references (leaves 37-54).