Toxicity of oiled wetland sediments influenced by natural and enhanced bioremediation /

A multi-phase bioremediation project was initiated raphics.

Bibliographic Details
Main Author: Mueller, Danica Christine
Format: Thesis eBook
Language:English
Published: [Place of publication not identified] : [publisher not identified] ; 1998.
Subjects:
Online Access:Link to OAKTrust copy
Description
Summary:A multi-phase bioremediation project was initiated raphics.
alternate electron acceptor. The effects of treatments
amendments to evaluate their effects on
amphipod and P450 RGS assays were sensitive to
amphipod, cytochrome P450 Reporter Gene System (RGS),
analyses of sediment elutriates revealed that Microtox
and decreased significantly over time. However, a
and its associated wetlands were set aside for
application of weathered oil and selected nutrient
associated with intrinsic petroleum degradation. Acute
bioremediation. The amendments evaluated were
chl.omol3adTM and Salmonella assays were not sensitive
conclusion, significant toxicity was detected in oiled
consistently alter sediment-associated toxicity with
December of 1994 and monitored sediment toxicity
during extensive flooding of the San Jacinto River
elevated near the mouth of the cove and decreased to
experimental conditions.
following the rupture of several oil and gas pipelines
hydrocarbon (TPH), and gas chromatography-mass
identified between Microtox and GC-MS target analyse
inorganic nutrients and inorganic nutrients plus an
levels in total sediment extractions. Subsequent GC-MS
mortality was initially high for all oiled sediments
naphthalenes, phenanthrenes and dibenzothiophenes. In
near Houston, Texas, in October of 1994. A small cove
non-detectable levels within 4 months. Acute toxicity
on sediment toxicity and mutagenicity were assessed
petroleum bioremediation studies. Phase I began in
Phase 11 began in March 1996 with the controlled
plots receiving nutrient amendments after 140 days,
plots that were oiled followed by a rapid decrease
possibly due to elevated ammonia levels. The Toxi-
Salmonella assays were not sensitive under the
sediment elutriates from 21 plots around the study
sediments that decreased to near background levels
significant increase in mortality was observed in
Significant toxicity was detected (Microtox*) in all
site over a period of 7 months. Sediment toxicity was
spectrometry (GC-MS) target saturate concentrations.
that was correlated to petroleum losses. Amphipod
the exception of the amphipod assay. The Microtox,
to petroleum addition. Significant correlations were
Toxi-ChromoPad and Salmonella/microsome assays.
toxicity was evaluated using the Microtox 100% Test on
toxicity was strongly correlated with fluorenes,
using Microtox 100% and Solid-phase Tests and
was correlated with sediment total petroleum
weathered oil. However, the Toxi-ChromoPad and
within months. The addition of treatments did not
Item Description:"Major subject: Civil Engineering".
Vita.
Physical Description:x, 107 leaves : illustrations ; 28 cm.
Also available online.
Bibliography:Includes bibliographical references (leaves 78-87).