Homeobox genes expressed during Arabidopsis embryogenesis /
Homeobox genes are important developmental regulators. In animal systems they have been shown to be involved in regulating various aspects of embryogenesis. We are interested in investigating whether plant homeobox genes also play roles during plant embryo development. To this end, we have applied P...
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| Format: | Thesis Book |
| Language: | English |
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[Place of publication not identified] :
[publisher not identified] ;
1998.
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| Online Access: | http://proxy.library.tamu.edu/login?url=http://proquest.umi.com/pqdweb?did=732834201&sid=1&Fmt=2&clientId=2945&RQT=309&VName=PQD |
| Summary: | Homeobox genes are important developmental regulators. In animal systems they have been shown to be involved in regulating various aspects of embryogenesis. We are interested in investigating whether plant homeobox genes also play roles during plant embryo development. To this end, we have applied PCR techniques to isolate several homeodomain-containing genes from an Arabidopsis developing seed CDNA library. One clone, KNAT4II, was found to be preferentially expressed in Arabidopsis developing embryos. This suggested that KNAT4II may be involved in regulating Arabidopsis embryogenesis. In situ hybridization detected KNAT4II expression from octant stage through early torpedo stage embryos. When KNAT4II is ectopically expressed, it results in formation of abnormal flowers at low penetrable. Overexpression of KNAT4II in developing seeds produces embryos with increased numbers of cell layers, suggesting that KNAT4II may be involved in regulating cell division during early embryogenesis. A T-DNA insertion allele was isolated by screening the T-DNA tagged line collections. The T-DNA insertion site was found to be in the first intros and the insertion does not appear to cause any morphological defects. This suggests that other class 11 knotted-like genes may share redundant function with KNAT4II. |
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| Item Description: | Vita. |
| Physical Description: | ix, 135 leaves : illustrations ; 28 cm. Issued also on microfiche from University Microfilm Inc. |
| Bibliography: | Includes bibliographical references: pages 114-134. |