Molecular analysis of the genetic basis of natural disease resistance in cattle /
The demonstration of genetically based natural resistance brucellosis in cattle provides for innovative strategies in the control of zoonotic diseases that affect both cattle and people. In this model the bovine NRAMPI gene, the bovine homologue of a bovine gene (Bcg) controlling natural resistance...
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| Format: | Thesis Book |
| Language: | English |
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[Place of publication not identified] :
[publisher not identified] ;
1998.
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| Online Access: | http://proxy.library.tamu.edu/login?url=http://proquest.umi.com/pqdweb?did=732843251&sid=1&Fmt=2&clientId=2945&RQT=309&VName=PQD |
| Summary: | The demonstration of genetically based natural resistance brucellosis in cattle provides for innovative strategies in the control of zoonotic diseases that affect both cattle and people. In this model the bovine NRAMPI gene, the bovine homologue of a bovine gene (Bcg) controlling natural resistance to antigenically and taxonomically unrelated parasites, has been identified as major candidate gene controlling the resistant phenotype. We have ruled out a role for bovine NRAMPI in resistance/susceptibility to bovine tuberculosis by testing the association of the lesion and culture status of cattle naturally-exposed to Mycobacterium bovis with a genetic polymorphism in bovine NRAMPI . An in vitro model, in which the resistance- and susceptibility-associated alleles of bovine NRAMPI are expressed as transcends under the regulatory control of the bovine NRAMPI promoter in a murine macrophage cell line ['cgs], has been established to analyze the regulation of the NRAMPI gene and its role in macrophage function. The 5/ flanking region of bovine NRAMPI, despite the lack of TATA and CAAT boxes, contains a functional promoter and is capable of driving the expression of a transient in murine microphones. The bovine NRAMPI gene has been shown to be one of the genes involved the intercellular control of Salmonella dublin and Brucella amorous by microphones. A polymorphism within a microsatellite in the 3/ UTR of bovine NRAMPI critically affects the expression of the gene and the capacity of transferred vitro replication of Brucella but not Salmonella. Furthermore, NRAMPI may be involved in phagosome acidification and/or phagosome-lysosome fusion. Finally, we have adapted and optimized a set of molecular tools including the Differential Display Reverse Transcription Polymerase Chain Reaction (ddRT- PCR) and Reverse Transcription -1-7 RNA Polymerase- Dependent preliminary evidence suggests that bovine the process of phagocytosis of B. amorous and microphones to control the in Amplification (RT-TRDA) techniques for their use with bovine microphones to further our understanding of the genetic basis of natural disease resistance in cattle. |
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| Item Description: | Vita. |
| Physical Description: | xi, 127 leaves : illustrations ; 28 cm. Issued also on microfiche from University Microfilm Inc. |
| Bibliography: | Includes bibliographical references: pages 116-126. |