rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Developmentally regulated telomerase activity from Euplotes crassus
  name:
    name part: Bednenko, Janna.
    role:
      role term: creator
  type of resource: text
  genre: bibliography
  origin info:
    place:
      place term: xx
    date issued: 1998
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: print
    extent: xii, 176 leaves : illustrations ; 28 cm.
  abstract: Telomerase synthesizes telomeric DNA repeats via reverse transcription of a templating domain in its RNA subunit. It serves a dual role at telomeres, maintaining tracts of telomere repeats and forming telomeres de novo on broken chromosomes in a process called chromosome healing. In a ciliate organism E. crassus both reactions are readily observed. Vegetatively growing cells maintain pre-existing telomeres, while cells undergoing macronuclear development fragment their chromosomes and form telomeres de novo. This study provides the first evidence for developmentally regulated initiation of DNA synthesis by telomerase. In vitro, telomerase from developing E. crasser cells can add telomeric [G4T4] repeats directly onto non-telomeric primer 3' ends in a reaction mediated by a dissociable chromosome healing factor (CHF). Telomerase from vegetatively growing cells does not initiate synthesis directly onto non- telomeric DNA. Instead, it requires primers with at least four to five nucleotides of 3' terminal complementarity to the telomerase RNA template for efficient elongation. Initiation of non-telomeric 3' end extension occurs in the absence of Watson-crick base pairing between the primer 3' end and the telomerase RNA template. The instability of the primer-template interaction during extension of non- telomeric primer 3' end results in reiterative copying of C residues in the telomerase RNA templating domain if dTTP is omitted from telomerase reactions. Indiscriminate do addition observed under these conditions most likely occurs by a slippage mechanism described for other polymerizes and is confined to telomerase from developing cells. 80th developmental and vegetative forms of telomerase are associated with a DNA endonuclease that removes nucleotides from a primer 3' terminus prior to telomere repeat addition. Flexible positioning of the endonuclease site relative to the telomerase RNA template results in elimination of single nucleotide mismatches in do tracts of t…
  note: by Janna Bednenko.
  note: Vita.
  note: Issued also on microfiche from University Microfilm Inc.
  note: Ph. D. Texas A&M University 1998.
  note: Includes bibliographical references: pages 155-171.
  subject:
    topic: Major biochemistry
  identifier: 99-03033 UMI
  identifier: http://proxy.library.tamu.edu/login
  related item:
    location:
      url: http://proxy.library.tamu.edu/login
  record info:
    record content source: TXA
    record creation date: 990423
    record change date: 20190325093426.0
    record identifier: in00001479574