Beta transforming and fibroblast growth factors at the peri-implantation porcine conceptus-maternal interface /
High rates of porcine early embryonic loss occur during the first 25-30 days of gestation. The factors controlling this critical period of embryonic development are not known. Beta transforming growth factors ([TGFjs]) and fibroblast growth factors ([FGFs]) are peptide growth factors which appear to...
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| Format: | Thesis Book |
| Language: | English |
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[Place of publication not identified] :
[publisher not identified] ;
1998.
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| Online Access: | http://proxy.library.tamu.edu/login?url=http://proquest.umi.com/pqdweb?did=732837571&sid=1&Fmt=2&clientId=2945&RQT=309&VName=PQD |
| Summary: | High rates of porcine early embryonic loss occur during the first 25-30 days of gestation. The factors controlling this critical period of embryonic development are not known. Beta transforming growth factors ([TGFjs]) and fibroblast growth factors ([FGFs]) are peptide growth factors which appear to be key modulators of early embryonic development. Differential protein and mRNA expression of [TGF/S (TGFPI, TGFPZ and TGF;$3)] was observed in porcine conceptus trophectoderm, mesoderm, endoderm and embryonic ectoderm during peri-implantation period. [TGF/] receptors (Type-1 and Type 11) were also immunolocalized in porcine conceptuses at days 10 through 14 of gestation. The presence of [TGFPS] and their receptors indicates that porcine conceptuses are capable of synthesizing their own [TGFPS]. Intense [FGF], but not [aFGF], protein expression was detected in pea-implantation conceptuses, suggesting different roles of the related reptiles. Significant increase in [TGFPS, TGFP] receptors and [bFGF] protein expression in uterine endometrial tissues of pregnant, but not of cycling gilts, indicate pregnancy specific expression. It can be hypothesized that the presence of the concertos may be an important factor in controlling the expression of these molecules. The detected increase in uterine [TGFPS] protein expression concurs with the detected increase in [TGFPS] mRNA expression. [TGFPS] present in concertos and maternal cells could be important in conceptuamaternal signaling. Because [TGFPS] are secreted reptiles, analysis of uterine luminal bushings was done. All three [TGFP] isoforms were detected in uterine luminal Rushings at Days 10 through 14 of gestation. Bioactive [TGFPS] were detected in Day 12, 13 and 14 uterine luminal Rushings. Thus, active [TGFPS] at the conceptus- maternal interface may affect uterine luminal epithelial and concertos trophectoderm cells. The role of [TGFPI] in porcine concertos trophectoderm ECM and integer modulation was examined using trophoblastic vesicles generated from Day 12 porcine conceptuses. Results indicate that [TGFPI] increases FN expression in trophectoderm cells. No change was detected expression of oncofetal fibronectin, integer (15 or p1. Thus, [GFPI] may play important roles in porcine concertos implantation via modulating expression of the extracellular matrix protein fibronectin. |
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| Item Description: | Vita. |
| Physical Description: | xv, 189 leaves : illustrations ; 28 cm. Issued also on microfiche from University Microfilm Inc. |
| Bibliography: | Includes bibliographical references: pages 162-187. |