Organization and expression of bovine MHC (BoLA) class I genes /
The MHC class I genes represent a fascinating yet incompletely understood region of the MI-IC and the immune system. In the ovine MI-IC (BoI-A), very little is known abut the physical organization of the class I is known abut the physical organization of the class I identify the number of BOLA clas...
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| Format: | Thesis Book |
| Language: | English |
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[Place of publication not identified] :
[publisher not identified] ;
1998.
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| Online Access: | http://proxy.library.tamu.edu/login?url=http://proquest.umi.com/pqdweb?did=732834321&sid=1&Fmt=2&clientId=2945&RQT=309&VName=PQD |
| Summary: | The MHC class I genes represent a fascinating yet incompletely understood region of the MI-IC and the immune system. In the ovine MI-IC (BoI-A), very little is known abut the physical organization of the class I is known abut the physical organization of the class I identify the number of BOLA class 1 genes and how they are organized and expressed in the ovine genome. The first goal was to identify putative transcription factor binding sites and other regulatory signals which may explain class 1 transcription profiles among different class I loci and to examine whether 59 flanking sequences can be used to establish orthologous relationships between ovine and human and/or mice class I loci. The second goal was to construct a contiguous molecular map of the bovine MI-IC. The 5' finking regions of BOLA class I genes hold many regulatory signals which may control the differential transcription and expression of these genes. One newly identified conserved sequence upstream of the enhancer A element should be examined determine if it plays a role in regulation of these genes. The 5' finking sequences were not useful for determining orthology but they did show locus-specific conservation as did intros A sequences examined; consequently, these sequences may be useful for locus identification. Additionally, the genomic sequence of one allele (BSA) of BOLA locus A was determined and is the first description of the genomic sequence of a bovine classical class I gene. Finally, a contig of the BOLA class I region was constructed from overlapping BAC clones. Analysis of the gene content of the contig reveals a large number of non-classical class 1 genes but none of the classical class I genes. However, this contig presents a framework on which other BOLA class 1 studies can build to construct a complete, detailed molecular map of the region. |
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| Item Description: | Vita. In title symbols are used. |
| Physical Description: | xii, 160 leaves : illustrations ; 28 cm. Issued also on microfiche from University Microfilm Inc. |
| Bibliography: | Includes bibliographical references: pages 110-117. |