The effect of nitric oxide on in vitro release of GnRH from the bovine infundibulum /
Nitric oxide (NO) has been shown to be involved in the ics.
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| Format: | Thesis Book |
| Language: | English |
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[Place of publication not identified] :
[publisher not identified] ;
1998.
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| Subjects: |
| Summary: | Nitric oxide (NO) has been shown to be involved in the ics. release of GnRH from the hypothalamus of male rats. To determine involvement of NO in GnRH release in the ovine species, a study was conducted to determine the effect of exogenous NO on GGH release from ovine infundibular exploits incubated in vitro. Infundibula were collected from nth steers and heifers following slaughter at the Texas A&M University Rosenthal Meat Science Center. Infundibular exploit halves were randomly assigned to control or treatment groups. Explants were incubated in Krebs Ringer bicarbonate medium (>B). Samples of incubate (500 [Ptsl] were collected every 30 minutes and replaced with fresh media. At 120 minutes, 500 AM sodium nitroprusside (NP), a NO donor, was added to the exploits assigned to the treatment group. GnRH release from steer infundibular exploits at 180 minutes was greater (>0.05) in the NP treated group then the control group. In contrast to steers. 500 FM NP did not affect (p>0.05) Grill release from heifer infundibular exploits. An experiment was conducted to test the effect of several doses of NP on GnRH release from heifer infundibula. Neither 250 nor 750 AM NP altered GGH release from the heifer exhalants. To test the hypothalamas that absorption of exogenous NO with hemoglobin (Hb), a NO scavenger, would block the action of NO on GOH release, steer experts were divided into three treatment groups: 500 gM NP, 500 AM NP plus Hb (10 g.g/mL), and Hb (10 pg/m1.) alone. At 180 minutes, GnRH release in the 500 AM NP + Hb group was Neater (>0.05) then in the Hb group. Heifer exploits were also assigned to the alive three treatment groups. No significant differences between treatment and control groups were observed in any of the time Griots. These experiments demonstrate that NP increases Grill release from infundibular exploits of steers, but not heifer infundibular exploits. These results suggest that the effect of NO on Grill release in cattle may Ix gender dependent. The failure of Hb to block NP-induced GnRH release may be due to an inappropriate dose for the tissues used in the study. |
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| Item Description: | "Major subject: Physiology of Reproduction". Vita. |
| Physical Description: | x, 65 leaves : illustrations ; 28 cm. |
| Bibliography: | Includes bibliographical references: pages 44-49. |