Table of Contents:
  • Getting started
  • Types of nerve cell cultures, their advantages and limitations
  • Primary dissociated cell cultures
  • Transfecting cultured neurons
  • Characterizing and studying neuronal cultures
  • Culture and experimental use of the PC12 rat pheochromocytoma cell line
  • Neuronlike cells derived in culture from P19 embryonal carcinoma and embryonic stem cells
  • Culturing the large neurons of aplysia
  • Culturing spinal neurons and muscle cells from Xenopus embryos
  • Cultures from chick peripheral ganglia
  • Culturing mammalian sympathoadrenal derivatives
  • Mass cultures and microislands of neurons from postnatal rat brain
  • Rat hippocampal neurons in low-density culture
  • Rat striatal neurons in low-density, serum-free culture
  • Cell culture of cholinergic and cholinoceptive neurons from the medial habenula
  • The Cerebellum: purification and coculture of identified cell populations
  • Organotypic slice cultures of neural tissue
  • Culture of astrocytes, oligodendrocytes, and O-2A progenitor cells
  • Tissue culture methods for the study of myelination.