rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Potential Oxidative Stress due to Pb Exposure
  name:
    name part: Elms, Rene' Davina.
    role:
      role term: creator
  name:
    name part: Elms, Rene' Davina.
    role:
      role term: creator
  name:
    name part: Funkhouser, Edward A.
    role:
      role term: degree supervisor.
  name:
    name part: Texas A & M University
    name part: University Undergraduate Research Fellows Program.
  type of resource: text
  genre: Undergraduate thesis
  origin info:
    place:
      place term: txu
    date issued: 1998
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: print
    extent: 1 online resource (23 pages).
  abstract: The hazards of Pb exposure has been a topic of concern for many years. This research was developed to investigate the possibility of Pb induced oxidative stress. The research objectives were to observe Pb induced lipid peroxidation and Pb induced increases in oxidation of glutathione in K562 myelogenous leukemia cells at low Pb levels. The approach consisted of incubating K562 cells in solutions of 0, 300, and 700 ppb Pb dissolved in RPMI cell medium for a total of 96 hours. After this period of incubation aliquots of cells were taken, placed in clean medium, and incubated for 96 hours. Samples were taken at 24 and 96 hours of Pb exposure, and at 96 hours after cessation of Pb exposure, then subsequently assayed for lipid peroxidation and glutathione levels.
  abstract: Lipid peroxidation was determined by the detection of malondialdehyde (MDA) spectrophotometrically at 535nm by the Thiobarbituric acid (TBA) assay, while oxidized and reduced glutathione levels were determined spectrophotometrically at 412 nm. Results showed no change in MDA levels in treated cells as compared to the control. The GSH:GSSG ratios were significantly greater for treated cells at 96 hours of exposure as compared to the controls, indicating a possible compensatory response.
  target audience: specialized
  note: "Major Subject: Biochemistry/Chemistry".
  note: Digitized from print version held at Pickle Center High Density Storage, barcode 24829659
  note: Fellows Thesis Texas A&M University 1998.
  note: Includes bibliographical references: leaves 15-18.
  note: HDR
  subject:
    topic: Major biochemistry/chemistry
  subject:
    topic: glutathione
  identifier: http://hdl.handle.net/1969.1/ETD-TAMU-1998-Fellows-Thesis-E47
  identifier: https://hdl.handle.net/1969.1/ETD-TAMU-1998-Fellows-Thesis-E47
  related item:
    location:
      url: http://hdl.handle.net/1969.1/ETD-TAMU-1998-Fellows-Thesis-E47
  record info:
    record content source: TXA
    record creation date: 231116
    record change date: 20151021053410.0
    record identifier: in00001465122