rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Mycoparasitic role of extracellular chitinase in Trichoderma virens
  name:
    name part: Baek, Jong-Min
    name part: 1966-
    role:
      role term: creator
  type of resource: text
  genre: bibliography
  origin info:
    place:
      place term: xx
    date issued: 1998
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: print
    extent: xii,120 leaves : illustrations ; 28 cm.
  abstract: Quantitative competitive polymerase chain reaction (QC-PCR) was developed for specific detection and enumeration of genetically modified strains of Trichoderma virens in the soil environment. The PCR technique was much more sensitive than the traditional dilution plating method especially for the detection of fungal spores. An efficient procedure for extraction of DNA from nonsterile soil was determined. The procedure, a combination of SDS-heating and bead-beating, resulted in a 85-95% relative efficiency of recovery of T.virens DNA from soil samples. This QCPCR technique was also used to study the interaction between two strains of T. virens in which a chitinase gene was either disrupted or overexpressed. A homologous transformation system was developed for T. virens based on complementation of an arginine auxotrophic strain. The mutation was found in the arg2 gene encoding the small subunit of carbamoyl phosphate synthetase. The wild-type arg2 gene was cloned and used as a selectable marker in a transformation system that yielded as many as 370 transformants per mg of DNA. The predicted amino acid sequence of the arg2 gene showed 56 - 82% identity with homologous polypeptides of Neurospora crassa, Magnaporthe grisea and yeast. A 42-kDa extracellular chitinase was found to be the major source of endochitinase activity when T. virens was cultured in a fungal cell wall medium. The role of this extracellular chitinase in the biocontrol activity and rhizosphere competence of T. virens was determined. The gene encoding the 42-kDa extracellular chitinase was cloned from T. virens and disrupted (KO) or constitutively overexpressed (COE) in the fungus through genetic transformation. KO and COE transformants were selected for similarity to the wildtype strain in growth rate, sporulation and antibiotic production. These strains demonstrated similar colonization efficiency on cotton roots and growth/survival in nonsterile soil amended with different carbon sources. However, K…
  note: by Jong-Min Baek.
  note: Vita.
  note: "Major Subject: Plant Pathology".
  note: Issued also on microfiche from University Microfilms Inc.
  note: Ph. D. Texas A&M University 1998.
  note: Includes bibliographical references: pages 105-119.
  subject:
    topic: Major plant pathology
  identifier: 98-30871 UMI
  identifier: http://proxy.library.tamu.edu/login
  related item:
    location:
      url: http://proxy.library.tamu.edu/login
  record info:
    record content source: TXA
    record creation date: 990128
    record change date: 20190325093405.0
    record identifier: in00001463834