Radiation hybrid mapping of bovine chromosome one /
steadily increased over the last fifteen years produce foundation genetic information that contribute significantly livestock generics in years come. Livestock geneticists, although unable to tap the financial resources available to those involved in the Human Project and Mouse Genome Projects, have...
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| Format: | Thesis Book |
| Language: | English |
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[Place of publication not identified] :
[publisher not identified] ;
1998.
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| Subjects: | |
| Online Access: | http://proxy.library.tamu.edu/login?url=http://proquest.umi.com/pqdweb?did=732548281&sid=1&Fmt=2&clientId=2945&RQT=309&VName=PQD |
| Summary: | steadily increased over the last fifteen years produce foundation genetic information that contribute significantly livestock generics in years come. Livestock geneticists, although unable to tap the financial resources available to those involved in the Human Project and Mouse Genome Projects, have nonetheless been able to generate genetic information on domestic farm animals using some the same technologies and the vast amounts of DNA sequence information provided by those projects. Gene mapping in cattle has progressed in recent history from constructing maps based on few isozymes using FISH, linkage, YACS, and BACS order loci and assign coding sequences to specific regions on chromosomes. Current gene maps cattle can provide useful information for quantitative trait loci searching, marker assisted selection, and identification of evolutionarily conserved genes from human or mouse for comparative mapping studies of chromosomal rearrangements and conserved sentences. Radiation Hybrid mapping has been an extremely powerful tool for constructing ordered maps of DNA sequences the human genome, and is now becoming an equally powerful tool mapping the bovine genome. As universal translator between cattle linkage maps, cattle cytogenetic maps, and human transcription maps, bovine radiation hybrid mapping data will valuable in order to exploit of the information these other resources can provide. This dissertation describes the development and initial characterization of 5000 rad and 12,000 bovine whole genome-radiation hybrid cell panels. These panels were used construct radiation hybrid map BTAI, including framework map ordered with 1000:1 odds, and a comprehensive map including all of the markers genotypes. Evolutionarily conserved genes were typed on human and bovine RH panels in order to produce parallel radiation hybrid maps. These maps revealed segments homologous HSAZI flanking segments homologous to HSA3 on BTAI. Microsatellites ordered on the RH map showed variation the [cRsooo/cM] distance ratio when compared with linkage data. The 12,000 rad panel was further characterized by typing centromeric markers and comparing the data with the 5000 rad data. |
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| Item Description: | Vita. "Major Subject: Genetics". |
| Physical Description: | xiii, 125 leaves : illustrations ; 28 cm. Issued also on microfiche from University Microfilm Inc. |
| Bibliography: | Includes bibliographical references: pages 116-123. |