A class of novel hydrophilic charged cyclodextrins for capillary electrophoresis /
A large number of chiral resolving agents have been used in capillary electrophoresis (CE). Unfortunately, most of them are mixtures which preclude a systematic approach to designing electrophoretic separations. Two new, high-purity, single-isomer, fully-charged cyclodextrins, namely, heptakis-(2,...
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| Format: | Thesis Book |
| Language: | English |
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[Place of publication not identified] :
[publisher not identified] ;
1998.
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| Subjects: | |
| Online Access: | http://proxy.library.tamu.edu/login?url=http://proquest.umi.com/pqdweb?did=737707971&sid=1&Fmt=2&clientId=2945&RQT=309&VName=PQD |
| Summary: | A large number of chiral resolving agents have been used in capillary electrophoresis (CE). Unfortunately, most of them are mixtures which preclude a systematic approach to designing electrophoretic separations. Two new, high-purity, single-isomer, fully-charged cyclodextrins, namely, heptakis-(2,3-diacetyl-6-sulfato)-[ beta] cyclodextrin and heptakis-6-sulfato-[beta]-cyclodextrin have been synthesized. The materials were characterized by indirect UV detection CE, NMR, and ESI-mass spectrometry. They were then tested on a broad range of chiral analytes. Their separation efficacy was tested at two limiting pH values, as required by the CHArged Resolving Agent Model (CHARM), with the selected chiral analytes at varying cyclodextrin concentrations. Separation selectivity was measured in the charged cyclodextrin-containing background electrolyte solutions using the "sandwich plug" approach. The effects of a givencharged cyclodextrin on separation selectivity are in agreement with the theoretical predictions of the CHARM model. Next, the effects of electroosmotic flow and background electrolyte electrolyte composition on peak resolution were examined. This dissertation will study the analytical utility of these chiral resolving agents and will discuss the theoretical, as well as practical considerations in designing separations using charged cyclodextrins. In addition, the importance of developing a well-defined and systematic approach to designing the best possible chiral separation conditions, as opposed to method development governed by trial and error, will be demonstrated. |
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| Item Description: | Vita. "Major Subject: Chemistry". |
| Physical Description: | xiii, 140 leaves : illustrations ; 28 cm. Issued also on microfiche from University Microfilms Inc. |
| Bibliography: | Includes bibliographical references: pages 120-124. |