http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
Non-invasive detection of protein kinase C Beta II and [], putative biomarkers for colon cancer using fecal messenger RNA
(p=0.025). We propose that expression of PKC [ ] and the
(PKC) activity and isozyme expression, which may influence
[ ]mMRNA expression, data were also expressed as a ratio
between PKC [ ]and [ ]. The ratio of PKC [ ]was
carcinogen on PKC [ ],, and [ ] mRNA levels in feces was
compared to animals without tumors, 0.50 ︢0.16
detect potential markers for colon cancer. Weanling rats
determined in order to develop non-invasive methodology to
development of colon cancer. It has recently been shown that
dietary fat and fiber can alter colonic protein kinase C
examined in this study. In addition, the effect of a
factorial design. Fresh fecal samples (n=6 per treatment)
Fecal PKC mRNA isozyme expression was altered by the
having 2.5-fold lower PKC @ expression compared to saline
higher in tumor bearing animals as compared to non-
incidence exerts a reciprocal effect on fecal PKC [ ], and
increased mucosal PKC [ ] MRNA expression compared with
injected animals. Fecal PKC 01, mRNA expression was 3-fold
injection of carcinogen (p < 0.03), with AOM injected animals
mayserve as non-invasive markers for colon carcinogenesis.
mucosal PKC [ ], expression was observed. Since tumor
or azoxymethane (AOM; 15 mg/kg body weight) in a 2 x 2 x 2
pectin and injected twice at one-week intervals with saline
polymerase chain reaction (RT-PCR) performed using primers
RNA was isolated and quantitative reverse transcriptase-
saline controls. No main effect of tumor incidence on
significantly higher in animals with tumors, 2.18 ︢1.25, as
specific for the regulatory subunit of PKC [ ], and [ ].
the expression of PKC [ ], and [ ]in fecal material was
the malignant transformation process. The effect of diet on
thedetection of colon cancer is of importance since early
There is strong evidence that select dietary factors play
tumor bearing animals (p < 0.03). In addition, AOM injection
were collected 36 weeks after the second injection, poly A+
were fed diets containing corn oil or fish oil, cellulose or
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Includes bibliographical references: pages 41-49.