Non-invasive detection of protein kinase C Beta II and [], putative biomarkers for colon cancer using fecal messenger RNA /
(p=0.025). We propose that expression of PKC [ ] and the
| Main Author: | |
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| Format: | Thesis eBook |
| Language: | English |
| Published: |
[Place of publication not identified] :
[publisher not identified] ;
1997.
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| Subjects: | |
| Online Access: | Link to OAKTrust copy |
| Summary: | (p=0.025). We propose that expression of PKC [ ] and the (PKC) activity and isozyme expression, which may influence [ ]mMRNA expression, data were also expressed as a ratio a key role in the A sensitive technique for between PKC [ ]and [ ]. The ratio of PKC [ ]was carcinogen on PKC [ ],, and [ ] mRNA levels in feces was compared to animals without tumors, 0.50 ︢0.16 detect potential markers for colon cancer. Weanling rats determined in order to develop non-invasive methodology to development of colon cancer. It has recently been shown that diagnosis can substantially dietary fat and fiber can alter colonic protein kinase C examined in this study. In addition, the effect of a factorial design. Fresh fecal samples (n=6 per treatment) Fecal PKC mRNA isozyme expression was altered by the having 2.5-fold lower PKC @ expression compared to saline higher in tumor bearing animals as compared to non- incidence exerts a reciprocal effect on fecal PKC [ ], and increased mucosal PKC [ ] MRNA expression compared with injected animals. Fecal PKC 01, mRNA expression was 3-fold injection of carcinogen (p < 0.03), with AOM injected animals mayserve as non-invasive markers for colon carcinogenesis. mucosal PKC [ ], expression was observed. Since tumor or azoxymethane (AOM; 15 mg/kg body weight) in a 2 x 2 x 2 pectin and injected twice at one-week intervals with saline polymerase chain reaction (RT-PCR) performed using primers ratio of [ ]in feces reduce mortality. RNA was isolated and quantitative reverse transcriptase- saline controls. No main effect of tumor incidence on significantly higher in animals with tumors, 2.18 ︢1.25, as specific for the regulatory subunit of PKC [ ], and [ ]. the expression of PKC [ ], and [ ]in fecal material was the malignant transformation process. The effect of diet on thedetection of colon cancer is of importance since early There is strong evidence that select dietary factors play tumor bearing animals (p < 0.03). In addition, AOM injection were collected 36 weeks after the second injection, poly A+ were fed diets containing corn oil or fish oil, cellulose or |
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| Item Description: | "Major subject: Nutrition". In title, symbols are used. Vita. |
| Physical Description: | x, 83 leaves : illustrations ; 28 cm. Also available online. Issued also on microfiche from Lange Micrographics. |
| Bibliography: | Includes bibliographical references: pages 41-49. |