Isolation and characterization of tomato genomic sequences related to fruit ripening /

a map-based cloning strategy was used. This strategy

Bibliographic Details
Main Author: Nah, Gyoungju, 1968-
Format: Thesis eBook
Language:English
Published: [Place of publication not identified] : [publisher not identified] ; 1997.
Subjects:
Online Access:Link to OAKTrust copy

MARC

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035 |a (OCoLC)40100552 
040 |a TXA  |c TXA  |d UtOrBLW 
049 |a TXAM  |a TXAR 
099 |a 1997  |a Thesis  |a N34 
100 1 |a Nah, Gyoungju,  |d 1968- 
245 1 0 |a Isolation and characterization of tomato genomic sequences related to fruit ripening /  |c by Gyoungju Nah. 
264 1 |a [Place of publication not identified] :  |b [publisher not identified] ;  |c 1997. 
300 |a x, 77 leaves :  |b illustrations ;  |c 28 cm. 
336 |a text  |b txt  |2 rdacontent 
337 |a computer  |b c  |2 rdamedia 
338 |a online resource  |b cr  |2 rdacarrier 
500 |a "Major subject: Plant Physiology". 
500 |a Vita. 
502 |b M.S.  |c Texas A&M University  |d 1997. 
504 |a Includes bibliographical references: pages 67-76. 
520 |a a map-based cloning strategy was used. This strategy  
520 |a accompanied by marked production of ethylene during ripening.  
520 |a are characterized by a dramatic increase in respiration  
520 |a around 30 kb in a cosmid contig. Two additional cosmid  
520 |a biology. Ripening patterns, based on respiratory behavior of  
520 |a Candidate YACs were recovered by others and here physical  
520 |a climacteric. Tomato is an example of climacteric fruit which  
520 |a clone has been initiated. 
520 |a clones obtained by cosmid library screening, 5Bl2 and llA5,  
520 |a conducted to estimate the kilobase distance among four DNA  
520 |a conducted. For chromosome walking, a candidate rin- 
520 |a containing YAC, designated Yrinl2 was subcloned and ligated  
520 |a designed from the YAC vector adjacent to the tomatok insert  
520 |a expect to contain the tomato rin locus has been physically  
520 |a Fruit ripening is a unique process in plant developmental  
520 |a fruit, are classified into two groups: climacteric and non- 
520 |a genomic DNA. In summary, a tomato YACclone (Yrinl2) which we  
520 |a includes genetic mapping, physical mapping, and chromosome  
520 |a indicating if the mutation resulting in rin is a chromosome  
520 |a into a cosmid vector to make a representative library. This  
520 |a library was screened with four markers (YrinllR, Yrinl2R,  
520 |a library. In attempting to isolate a tomato YAC end as a  
520 |a mapped, and construction of a cosmid contig representing the  
520 |a mapping, fine mapping, and chromosome walking have been  
520 |a marker more closely linked to rin than any currently  
520 |a markers (Yrin2R, YrinlIR, 45Dll, and pYAC4R) on Yrinl2. A  
520 |a maximum of 110 kb between Yrin2R and pYAC4R of which 30 kb  
520 |a narrow region around the rin locus. Physical mapping was  
520 |a physical map around the rin locus was generated suggesting a  
520 |a rearrangement, such as a deletion, it must be within a very  
520 |a recovered by false signals from either bacterial DNA or yeast  
520 |a recovered by YrinllR, Shcl, and Shc7 were aligned and span  
520 |a rin (ripening inhibitor) is a single locus tomato mutation  
520 |a RinlRin and rinlrin. No polymorphisms were detected  
520 |a ripening at the mature green stage. To isolate the rin gene,  
520 |a screen a tomato YAC library to obtain candidate YAC clones.  
520 |a Shcl, and Shc7) tightly linked to the rin locus. Positives  
520 |a showing non-climacteric development which essentially arrests  
520 |a terminus. However, this clone seems to be an artifact  
520 |a tomato DNA sequences contained in this high molecular weight  
520 |a walking. DNA markers in the region of rin were used to  
520 |a was spanned by chromosome walking using the Yrinl2 cosmid  
520 |a were used as probes for the investigation of RFLPs between  
530 |a Also available online. 
530 |a Issued also on microfiche from Lange Micrographics. 
650 4 |a Major plant physiology. 
856 4 1 |u https://hdl.handle.net/1969.1/ETD-TAMU-1997-THESIS-N34  |z Link to OAKTrust copy  |t 0 
999 |a MARS 
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952 f f |p noncirc  |a Texas A&M University  |b College Station  |c Cushing Memorial Library & Archives  |s cush tdrm  |d Cushing: Theses & Dissertations Microforms (Does not check out)  |t 0  |e 1997 Thesis N34  |h Other scheme  |i computer -- online resource 
952 f f |a Texas A&M University  |b College Station  |c Electronic Resources  |s www_evans  |d Available Online  |t 0  |e 1997 Thesis N34  |h Other scheme 
998 f f |a 1997 Thesis N34  |t 0  |l Cushing: Theses & Dissertations Microforms (Does not check out) 
998 f f |a 1997 Thesis N34  |t 0  |l Available Online