Isolation and characterization of tomato genomic sequences related to fruit ripening /
a map-based cloning strategy was used. This strategy
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| Format: | Thesis eBook |
| Language: | English |
| Published: |
[Place of publication not identified] :
[publisher not identified] ;
1997.
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| Subjects: | |
| Online Access: | Link to OAKTrust copy |
| Summary: | a map-based cloning strategy was used. This strategy accompanied by marked production of ethylene during ripening. are characterized by a dramatic increase in respiration around 30 kb in a cosmid contig. Two additional cosmid biology. Ripening patterns, based on respiratory behavior of Candidate YACs were recovered by others and here physical climacteric. Tomato is an example of climacteric fruit which clone has been initiated. clones obtained by cosmid library screening, 5Bl2 and llA5, conducted to estimate the kilobase distance among four DNA conducted. For chromosome walking, a candidate rin- containing YAC, designated Yrinl2 was subcloned and ligated designed from the YAC vector adjacent to the tomatok insert expect to contain the tomato rin locus has been physically Fruit ripening is a unique process in plant developmental fruit, are classified into two groups: climacteric and non- genomic DNA. In summary, a tomato YACclone (Yrinl2) which we includes genetic mapping, physical mapping, and chromosome indicating if the mutation resulting in rin is a chromosome into a cosmid vector to make a representative library. This library was screened with four markers (YrinllR, Yrinl2R, library. In attempting to isolate a tomato YAC end as a mapped, and construction of a cosmid contig representing the mapping, fine mapping, and chromosome walking have been marker more closely linked to rin than any currently markers (Yrin2R, YrinlIR, 45Dll, and pYAC4R) on Yrinl2. A maximum of 110 kb between Yrin2R and pYAC4R of which 30 kb narrow region around the rin locus. Physical mapping was physical map around the rin locus was generated suggesting a rearrangement, such as a deletion, it must be within a very recovered by false signals from either bacterial DNA or yeast recovered by YrinllR, Shcl, and Shc7 were aligned and span rin (ripening inhibitor) is a single locus tomato mutation RinlRin and rinlrin. No polymorphisms were detected ripening at the mature green stage. To isolate the rin gene, screen a tomato YAC library to obtain candidate YAC clones. Shcl, and Shc7) tightly linked to the rin locus. Positives showing non-climacteric development which essentially arrests terminus. However, this clone seems to be an artifact tomato DNA sequences contained in this high molecular weight walking. DNA markers in the region of rin were used to was spanned by chromosome walking using the Yrinl2 cosmid were used as probes for the investigation of RFLPs between |
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| Item Description: | "Major subject: Plant Physiology". Vita. |
| Physical Description: | x, 77 leaves : illustrations ; 28 cm. Also available online. Issued also on microfiche from Lange Micrographics. |
| Bibliography: | Includes bibliographical references: pages 67-76. |