A new approach for the identification and cloning of genes /
Cloning of exogenous DNA into bacterial artificial chromosomes (BACs) provides a new approach to the analysis of the genomes of higher organisms because BAC libraries can be constructed simply and analyzed efficiently. BAC libraries containing large genomic DNA inserts have become important tools f...
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| Format: | Thesis Book |
| Language: | English |
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[Place of publication not identified] :
[publisher not identified] ;
1997.
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| Online Access: | http://proxy.library.tamu.edu/login?url=http://proquest.umi.com/pqdweb?did=736824181&sid=1&Fmt=2&clientId=2945&RQT=309&VName=PQD |
| Summary: | Cloning of exogenous DNA into bacterial artificial chromosomes (BACs) provides a new approach to the analysis of the genomes of higher organisms because BAC libraries can be constructed simply and analyzed efficiently. BAC libraries containing large genomic DNA inserts have become important tools for positional cloning, physical mapping and genome sequencing. To facilitate ongoing genomics projects, methods were tested and developed to routinely construct and screen large insert BAC libraries for crop plants and model organisms. In this study, representative BAC libraries were constructed for Arabidopsis thaliana, rice, cotton and the filamentous fungus Ashbya gossypii. The Arabidopsis BAC library, in particular, has served as an important international genetic resource for sequencing the entire genome of a flowering plant. A method to achieve site-directed integration of BAC-size DNA into the genome of plants and fungi was developed using the Cre-lox site-specific recombination system. pBACwich was engineered to integrate into a lox site located on a plant chromosome by Cre-lox site-specific recombination. By introducing all candidate BAC clones into the same genomic locus, site-specific integration of large genomic clones may reduce the variability in gene expression caused by 'position effects'. This technology could potentially streamline the gene identification step of positional cloning. New knowledge of synteny promises to unify genetics and to affect our perception of higher order genome structure. To investigate a possible syntenic relationship between Arabidopsis and tomato, a set of cDNA clones (C 11 and C34: putative tomato jointless genes) were genetically mapped in Arabidopsis using recombinant inbred lines. In tomato, the cDNAs C 11 and C34 cosegregate on chromosome XI within a physical distance of 50 kb. Both C34 and C 11 were mapped on the same chromosome V of Arabidopsis, but unlike the tight linkage in tomato, both cDNAs were approximately 30 cM apart. |
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| Item Description: | Vita. "Major Subject: Genetics". |
| Physical Description: | xii, 167 leaves : illustrations ; 28 cm. Issued also on microfiche from University Microfilms Inc. |
| Bibliography: | Includes bibliographical references: pages 146-166. |