Characterization of a cDNA related to the tomato jointless locus /
The sequence analysis of tomato cDNA c34, a candidate gene
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| Format: | Thesis Book |
| Language: | English |
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[Place of publication not identified] :
[publisher not identified] ;
1997.
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| Online Access: | http://proxy.library.tamu.edu/login?url=http://proquest.umi.com/pqdweb?did=736824001&sid=1&Fmt=2&clientId=2945&RQT=309&VName=PQD |
| Summary: | The sequence analysis of tomato cDNA c34, a candidate gene for the jointless gene from tomato, revealed that the deduced amino acid sequence encoded 506 amino acid residues and contained two putative activation domains of transcription factors: one serine/threonine rich region and one acidic region. The gene family of c34 is present in distantly related species, including Arabidopsis thaliana, Nicotiana tabacum, Oryza sativa, and Pisum sativum. The sense c34 construct failed to complement the jointless mutant. Surprisingly, a transient epinasty growth phenotype was observed in young tomato in both sense and antisense c34- transformed young wild-type tomato plants. In c34- transformed tobacco, overproduction of the c34 transcript caused pleiotropic alterations in the mature tobacco plants, which include reduced plant height, decreased elongation of peduncle and corolla, and suppressed pigmentation of petals. In c34-transformed Arabidopsis, an incidence of a gene silencing effect between two homologous genes was observed. By impairing the host gene ATTS2203 with its potential tomato homolog c34 in Arabidopsis, the sense and antisense c34- transformed Arabidopsis exhibited conspicuous alterations in the inflorescence structure such that the spiral phyllotaxy was converted to the whorled phyllotaxy, and the inflorescence growth pattern was switched from indeterminate to determinate by producing terminal flowers at the inflorescence apex. Based on the evidence of sequence analyses and transgenic assays of tomato, tobacco, and Arabidopsis, I infer that c34 might function as a transcription factor involved in controlling hormone perception, translocation, or an appropriate hormone ratio. In Arabidopsis, the c34 homologue plays a notable role in the early stages of flower development. |
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| Item Description: | Vita. "Major Subject: Genetics". |
| Physical Description: | ix, 99 leaves : illustrations ; 28 cm. Issued also on microfiche from University Microfilms Inc. |
| Bibliography: | Includes bibliographical references: pages 88-98. |