An analysis of the immune response elicited in the equine by ERA and Trimune rabies vaccines as determined by the rapid flourescent focus inhibition test (RFFIT) and by the enzyme-linked immunosorbent assay (ELISA) /

The serum antibody response elicited in the equine, over a five-year period, when vaccinated with either a modified-live (ERA) or an inactivated (Trimune) rabies vaccine was evaluated by the RFFIT and the ELISA. The ELISA, utilizing whole virion inactivated rabies vaccine for the coating antigen, w...

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Bibliographic Details
Main Author: Barnes, Nancy Batterton
Format: Thesis Book
Language:English
Published: [Place of publication not identified] : [publisher not identified] ; 1997.
Subjects:
Online Access:http://proxy.library.tamu.edu/login?url=http://proquest.umi.com/pqdweb?did=736823651&sid=1&Fmt=2&clientId=2945&RQT=309&VName=PQD

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099 |a 1997  |a Dissertation  |a B372 
100 1 |a Barnes, Nancy Batterton. 
245 1 3 |a An analysis of the immune response elicited in the equine by ERA and Trimune rabies vaccines as determined by the rapid flourescent focus inhibition test (RFFIT) and by the enzyme-linked immunosorbent assay (ELISA) /  |c by Nancy Batterton Barnes. 
264 1 |a [Place of publication not identified] :  |b [publisher not identified] ;  |c 1997. 
300 |a xi, 111 leaves :  |b illustrations ;  |c 28 cm. 
336 |a text  |b txt  |2 rdacontent 
337 |a unmediated  |b n  |2 rdamedia 
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504 |a Includes bibliographical references: pages 98-110. 
500 |a Vita. 
502 |b Ph. D.  |c Texas A&M University  |d 1997. 
500 |a "Major Subject: Veterinary Public Health". 
530 |a Issued also on microfiche from University Microfilms Inc. 
520 |a The serum antibody response elicited in the equine, over a five-year period, when vaccinated with either a modified-live (ERA) or an inactivated (Trimune) rabies vaccine was evaluated by the RFFIT and the ELISA. The ELISA, utilizing whole virion inactivated rabies vaccine for the coating antigen, was developed. This ELISA detected levels of the immunoglobulin lgG (H+L), the major immunoglobulin produced in response to infection with rabies virus. This ELISA appeared to be more sensitive than the RFFIT. The RFFIT detected levels of serum neutralizing antibodies, an indicator in vitro of the level of protection conferred by the immune response in vivo. The Trimune vaccine group produced higher levels of antibody in the first year. However, the ERA group demonstrated higher levels in the subsequent years. The modified-live vaccine (ERA) conferred a 100% level of positive immunity during the last two years when no vaccination program was implemented. This evaluation of the immune response in the equine can serve as a guide for equine managers in implementing vaccination programs. The ELISA developed was successful in detecting antigen-antibody complexes prior to detection of neutralizing antibodies. This should prove useful as a diagnostic tool in the early detection of rabies infection. 
650 4 |a Major veterinary public health. 
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