Gene clusters and introns of Spirogyra maxima and other charophyte cpDNAs and their phylogenetic implications in green plants /
The gene clusters and introns in chloroplast DNAs of
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| Format: | Thesis Book |
| Language: | English |
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[Place of publication not identified] :
[publisher not identified] ;
1997.
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| Online Access: | http://proxy.library.tamu.edu/login?url=http://proquest.umi.com/pqdweb?did=736824431&sid=1&Fmt=2&clientId=2945&RQT=309&VName=PQD |
| Summary: | The gene clusters and introns in chloroplast DNAs of charophytes, the closest green algal group to land plants, are poorly known. Here, the genes of four operons unique to land plants (rpl23, clpP, psaA, and psbB operons) were investigated in Spirogyra cpDNA, and psbB operon genes were also investigated in Coleochaete and Chara cpDNAs. In addition, the presence/absence of introns in 6 genes (petB, petD, rpl2, rpl16, clpP, and ndhA) was determined in Spirogyra. Spirogyra contains three gene clusters (rpl23, clpP, and psaA) and 6 introns in 5 genes (petD, rpl2, rpl16, clpP, and ndhA), which are shared by land plants. These results support the placement of Spirogyra, along with other charophytes, close to the land plant lineage. However, unlike land plants, Spirogyra has the psbB operon genes in two separate gene clusters, and petD contains an intron but petB does not contain an intron. Unlike Spirogyra, Coleochaete and Chara cpDNAs contain a psbB gene cluster similar to land plants. Coleochaete contains introns in both petB and petD, and Chara has an unusually large petB intron but no intron in petD. The detailed characterization of the psbB gene cluster, its intron distribution, and sequence analysis using psbB, petB and petD gene sequences indicate that Coleochaete is most closely related to land plants, and that Spirogyra is basal to Chara, Coleochaete, and land plants. |
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| Item Description: | Vita. "Major Subject: Botany". |
| Physical Description: | ix, 95 leaves : illustrations ; 28 cm. Issued also on microfiche from University Microfilms Inc. |
| Bibliography: | Includes bibliographical references: pages 59-65. |