Complex regulation in the promoter of a developmentally regulated gene of Neurospora crassa /
The Neurospora crassa conidiation-specific gene, con-10,
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| Format: | Thesis Book |
| Language: | English |
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[Place of publication not identified] :
[publisher not identified] ;
1997.
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| Online Access: | http://proxy.library.tamu.edu/login?url=http://proquest.umi.com/pqdweb?did=736584501&sid=1&Fmt=2&clientId=2945&RQT=309&VName=PQD |
| Summary: | The Neurospora crassa conidiation-specific gene, con-10, encodes a small polypeptide that has homology with a general stress-induced gene, gsib, of Bacillus subtilis. The promoter region of con-10 contains two pairs of conserved sequence elements. The CRS-B (conidiation response sequence- B) and CGE (con-10 general enhancer) elements have been proposed to function as transcriptional enhancers. Both CRS- B elements are necessary for full activation of con-10, whereas CGEs are functionally redundant and one element is sufficient for maximal expression. CRS-B and CGE elements are not sufficient for developmental activation of a reporter gene. However, they appear to function as general enhancers that work in concert with unidentified developmental coactivator(s) during macroconidiation. con-10 is also highly expressed during microconidiation, a different type of asexual sporulation. CRS-B elements appear to have no significant role in con-10 expression during microconidiation. One copy of each class of enhancer element appears to contribute to expression of con-10 in response to light cues. rco-I is required for transcriptional repression of con-10 expression, and rco-I mutants are blocked in 'd'at'on in N. crassa at a late stage in development. RCOI has con-10 homology to Tupl of S. cerevisiae and appears to function as a repressor of genes during vegetative mycelial growth. In wild-type strains, prolonged starvation results in sporulation and con-10 expression. However, in the rco-I mutant strain, con-10 expression is rapidly and highly induced by carbon and nitrogen starvation and other stresses in the absence of development. Thus, in the wildtype, rco-I functions to repress con-10 expression in mycelia and to block induction of the gene in response to starvation and stress. |
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| Item Description: | Vita. "Major Subject: Plant Pathology". |
| Physical Description: | ix, 91 leaves : illustrations ; 28 cm. Issued also on microfiche from University Microfilms Inc. |
| Bibliography: | Includes bibliographical references: pages 78-90. |