Cloning, sequencing, expression and characterization of the feline CD28 / CD80 accessory signaling complex /

T cell activation is mediated through two distinct signaling

Bibliographic Details
Main Author:	Hash, Stephen Martin
Format:	Thesis Book
Language:	English
Published:	[Place of publication not identified] : [publisher not identified] ; 1997.
Subjects:	Major veterinary microbiology.
Online Access:	http://proxy.library.tamu.edu/login?url=http://proquest.umi.com/pqdweb?did=739887471&sid=1&Fmt=2&clientId=2945&RQT=309&VName=PQD

Description
Summary:	T cell activation is mediated through two distinct signaling events. In human and murine systems, it has been demonstrated that an antigen specific interaction mediated through recognition by the T cell receptor of antigen presented in the context of MHC delivers the primary signal, while the secondary signal is delivered by an antigen independent interaction (Allison and Lanier, 1987). The interaction between CD28 on the T cell and CD80 (B7-1) on the antigen presenting cell has been established as an essential element in the delivery of this secondary signal (Linsley et al., 1993). In rodents and primates, signals delivered through the cross-linking of CD28 by CD80 in conjunction with TCR mediated signaling leads to augmented T cell proliferation and cytokine production, while in the absence of the signal, TCR cross linking results in clonal anergy (Schwartz, 1992). Despite the importance of the CD28/CD80 interaction in the development of the immune response, these molecules have only been isolated from a limited number of species. CD28 has been cloned in rodent and primate species as well as the rabbit, with a seemingly analogous receptor obtained in the chicken. Conversely, CD80 has only been cloned in rodent and primate species. The feline system is evolutionarily divergent from primates and rodents. Due to the importance of the binding of CD28 and CD80 in the development and maintainence of a functional T cell response, these accessory molecules were cloned, their sequence determined and expressed to confirm the interaction. The isolation of these molecules will provide valuable markers for and mediators of the feline T cell response and might potentially establish an evolutionary intermediate to which previously cloned molecules might be compared. Further, the feline system is increasingly important as a potential model of human disease, specifically HIV, and a more complete understanding of the pathogenicity of retroviral infections in the cat may lead to answers about analogous diseases in other systems. The more readily that the immune system is characterized in the feline may increase the ease with which infections are understood in that system as well as others.
Item Description:	Vita. "Major Subject: Veterinary Microbiology". In title, numerals are used.
Physical Description:	xvii, 168 leaves : illustrations ; 28 cm. Issued also on microfiche from University Microfilms Inc.
Bibliography:	Includes bibliographical references: pages 141-167.