Identification and characterization of the Anabaena sp. strain PCC 7120 hupL DNA rearrangement /
Arg3O8 and Tyr433 prevented XisC from rearranging the
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| Format: | Thesis eBook |
| Language: | English |
| Published: |
[Place of publication not identified] :
[publisher not identified] ;
1997.
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| Subjects: | |
| Online Access: | Link to OAKTrust copy |
| Summary: | Arg3O8 and Tyr433 prevented XisC from rearranging the artificial substrate in E. coli. at least three programmed DNA rearrangements occur during bp direct repeats that flank the element. The predicted HupL differentiated cells specialized for nitrogen fixation. differentiation and involve the deletion of DNA elements from differentiation are uncommon and have been described in only during the late stages of heterocyst development. The element. Inactivation of xisc in the chromosome of Anabaena encodes the recombinase required for excision of the hupl Expression of xisc in E. coli is sufficient to cause site- heterocyst differentiation. Heterocysts are terminally HupTLTV operon and is thought to be involved in regulation of hydrogenase and to HupU. An open reading frame, named xisc, nitrogen fixation genes; hupl message was detected only PCC 7120 prevents rearrangement of the hupl element. polypeptide is homologous to the large subunit of [NiFe] predicted product of a gene located upstream of hupl, shows predicted XisC protein shows sequence similarity to XisA, the Programmed DNA rearrangements that occur during cellular rearrangement a 10.5-kb DNA element is excised from within recombinase required for excision of the nifd element, and to recombinases. Site-directed mutagenesis of the invariant sequence similarity to the small subunit of uptake specific rearrangement of an artificial substrate. The the catalytic domain of the Int family of site-specific the hupl gene by site-specific recombination between two 16- These rearrangements take place late during heterocyst two prokaryotic organisms. In Anabaena sp. strain PCC 7120 uptake hydrogenase. hupl is expressed similarly to the uptake hydrogenases and to HupU which forms part of the was identified near one end of the hupl DNA element and within the nifd, fdxn, and hupl genes. In the hupl |
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| Item Description: | "Major subject: Microbiology". In title, numerals and symbols are used. Vita. |
| Physical Description: | viii, 79 leaves : illustrations ; 28 cm. Also available online. Issued also on microfiche from Lange Micrographics. |
| Bibliography: | Includes bibliographical references. |