Effect of cholesterol on post-thaw motility and plasma membrane damage of equine spermatozoa /
(0. 125 mM) on post-thaw spermatozoal motility parameters
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| Format: | Thesis eBook |
| Language: | English |
| Published: |
[Place of publication not identified] :
[publisher not identified] ;
1997.
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| Subjects: | |
| Online Access: | Link to OAKTrust copy |
| Summary: | (0. 125 mM) on post-thaw spermatozoal motility parameters (p<0.05) incorporation of cholesterol at all concentrations 0.05) percentages of total, progressive and rapid and plasma membrane integrity. To evaluate membrane damage, and propidium iodide fluorescent stains. Addition of centrifugation extender (MK or TALP) and added cholesterol characteristics than TALP media. Cholesterol treatments cholesterol content with a cholesterol-methyl-p-cyclodextrin cholesterol to either extender significantly improved (p < complex. Experiment 1 was conducted to evaluate effects of computerized motility parameters. Two centrifugation media concentration on membrane uptake. There was significant effects of cholesterol incorporation into plasma membranes of ejaculates of three stallions. Four different concentrations equine spermatozoa using a novel method to alter membrane Experiment 3, cryoprotective effects of cholesterol on four cholesterol concentrations (0.0, 0. 125, 0.25 or 0. 5 from six stallions were cryopreserved to evaluate effect of in both extenders when compared to the control. In In Experiment 2, tritium-labeled cholesterol was utilized to incorporation also significantly decreased the number of incubation time tended to decrease percent spermatozoal incubation times. An incubation temperature of 24'C also MK (p<0.05) and TALP (p<0.06) media. MK or TALP media were used to evaluate effect of mM cholesterol (p < 0. 05). Cholesterol incorporation did mM) five incubation times (0, 15, 30, 45 or 60 minutes), and motility and preserved velocity parameters over longer motility. Incubation for 15 minutes tended to improve not affect velocity parameters (p>0.05). Increasing of tritiated cholesterol (0. 0, 0. 125, 0.25 or 0. 50 mM) in significant improvement in percent total motility with 0. 125 spermatozoa were stained with carboxyfluorescein diacetate spermatozoa with post-thaw damage to the plasma membrane in spermatozoal motility in post-thaw samples. Cholesterol spermatozoal plasma membranes were evaluated. Ejaculates sucrose extender (MK) and a modified Tyrode's medium (TALP). tended to improve post-thaw motility over control media, with tended to increase motility parameters when compared to 37'C. The MK media generally yielded better spermatozoal motility This study was designed to investigate the cryoprotective two incubation temperatures (24 or 37'C) on post-thaw verify spermatozoal incorporation of cholesterol in were used throughout the study: a non-fat skim milk glucose- |
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| Item Description: | "Major subject: Veterinary Medicine and Surgery". Vita. |
| Physical Description: | x, 58 leaves : illustrations ; 28 cm. Also available online. Issued also on microfiche from Lange Micrographics. |
| Bibliography: | Includes bibliographical references. |