Monosporascus root rot/vine decline : a study of double-stranded (DS) RNA and its role in the pathogenesis of Monosporascus cannonballus on muskmelon /
449+.
| Main Author: | |
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| Format: | Thesis eBook |
| Language: | English |
| Published: |
[Place of publication not identified] :
[publisher not identified] ;
1997.
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| Subjects: | |
| Online Access: | Link to OAKTrust copy |
| Summary: | 449+. accumulation) and hypovirulence (on muskmelon) in the fungus, aggressive isolate (Az9O-33-) in controlled pathogenicity and Tx93-314+'- caused less stunting than Az9O-33- and were Aniline blue staining of fungal mycelia in muskmelon associated with hypovirulence and/or culture degeneration. Ca9l-17 16+, Tx93-529+, and Tx93-314+/-. In contrast, Ca9l-17"+, Tx93-529+, and Tx93-314+'-) were examined in clones from the dsRNAs in the hypovirulent isolate, Tx93- cloning dsRNAs in M. cannonhallus will be used to derive CDNA degeneration (slow growth, reduced sporulation, and pigment determine if a specific set of dsRNA fragments were Double-strand (ds) RNA has been shown to induce culture generated from Tx93-529+. After introducing additional growth assays and accumulated a yellow to brown pigment. hypovirulent isolate was added to the inoculum mixture at a hypovirulent isolates (Tx93-449+, Ca9l-17 95+, and Tx9l-18@) Initially, only fungal ribosomal RNA-CDNA clones were isolate alone, suggesting that biocontrol of D might be isolate Tx93449 + was consistently hypovirulent and Az9O-33- isolates had a culture phenotype similar to that of wildtype isolates. In contrast, isolate Tx93-449+ grew significantly microplot trial suggest that virulence was reduced when the Monosporascus cannonballus. Five M cannonballus isolates other known dsRNA mycoviruses. The techniques developed for partially 17 , Tx93 hypovirulent. In addition, these pathogenicity data from seven trials, isolates Ca9l96+ -529+, possible. Two isolates (Tx93-529+ and Ca9l-17 96+) were purification techniques, nine CDNA clones (400 bp to 1500 bp) ratio 1: IO (virulent:hypovirulent). At this ratio, plants repeated greenhouse pathogenicity trials from 1995 to 1996 to seedlings infected with Tx93-449+ revealed that this isolate slower (LSD, P=0.05) than all other isolates in radial tests. Results from two greenhouse trials and one field There was some variation in virulence levels for isolates These clones are currently being sequenced and compared with tissue compared to the virulent isolate, Az9O-33-. Three took three times longer to penetrate and colonized root used to make a CDNA library derived from their dsRNAs. was consistently virulent on muskmelon. Pooling greenhouse were less stunted than those inoculated with the virulent were obtained from the dsRNAs associated with Ca9l-17 96+ . were tested for their ability to affect the virulence of an with different dsRNA banding patterns, (Az9O-33-, Tx93-449+, |
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| Item Description: | "Major subject: Plant Pathology". Vita. |
| Physical Description: | ix, 76 leaves : illustrations ; 28 cm. Also available online. Issued also on microfiche from Lange Micrographics. |
| Bibliography: | Includes bibliographical references: pages 68-75. |