Arginine metabolism in porcine enterocytes /
(1.34 ︢0.11, 58.6 ︢2.4, 15.2 ︢1.4 for NH4, HCO3-, and
| Main Author: | |
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| Format: | Thesis eBook |
| Language: | English |
| Published: |
[Place of publication not identified] :
[publisher not identified] ;
1997.
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| Subjects: | |
| Online Access: | Link to OAKTrust copy |
| Summary: | (1.34 ︢0.11, 58.6 ︢2.4, 15.2 ︢1.4 for NH4, HCO3-, and (ASL), and arginase were determined in the cytosol and (OAT) activities were measured in various tissues. P5C 0.01 for ATP, aspartate, and citrulline, respectively), and 5.5 for cytosol), OCT (5.13 ︢0.24 and 17.1 ︢1.2 for administration increased plasma concentrations of proline and had no effect on plasma concentrations of ornithine, Arginase was present in both the cytosol and mitochondria. arginine synthesis plays an important role in arginine arginine-min-'-mg protein-1 ), and arginase (68.1 + 4.5 nmol argininosuccinate synthase (ASS), argininosuccinate lyase argininosuccinate-min-l-mg protein-'), ASL (3.57 ︢0.24 nmol ASL (0.63 0.04) were higher than the hepatic enzymes with at 550C. Vmax.. values of CPS-1 (6.12 ︢0.25 nmol ATP, respectively), ASS (2.86 ︢0.16, 0.054 0.003, 0.15 ︢ carboxylate (P5C) synthase and ornithine aminotransferase citruiline. In 60-day-old fed pigs, gabaculine citrulline or arginine. The results suggest that endogenous citrulline-min-l-mg protein-'), ASS (1.91 ︢0.13 nmol enterocyte urea cycle enzymes are consistent with the role Enterocytes contain a functional urea cycle and may play a enterocytes. The administration of gabaculine to 60-day-old fasted pigs increased plasma concentrations of ornithine and for endogenous synthesis of arginine in pigs. Pyrroline-5- growing pigs. The activities of carbamoyl phosphate homeostasis in food-deprived 60-day-old pigs but not in fed mitochondria of enterocytes. CPS-1 and OCT were Mitochondrial arginase activity was decreased by preheating mitochondrial, whereas ASS and ASL were cytosolic enzymes. nmol citrulline-min-l-mg protein-') was similar to hepatic OCT. The substrate Km values (mmol/L) for arginase (91.3 ︢ of the small intestine in the net production of citrulline ornithine and carbamoyl phosphate, respectively), CPS-1 ornithine and citrulline from glutamine in isolated pigs. pivotal role in arginine metabolism and homeostasis in potent inhibitor of OAT and prevented net synthesis of proline but decreased plasma concentrations of arginine and synthase was detected only in jejunal enterocytes. OAT was synthase-1 (CPS-1), ornithine carbamoyltransferase (OCT), than those in mammalian liver. Vmax, of OCT (690.5 ︢36.3 the exception of CPS-1 for NH4+. The kinetic properties of ubiquitous in tissues. Gabaculine (Ki 19 ︢2 4mol/L) was a urea-min-l-mg protein-' in pre-heated cytosol) were lower |
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| Item Description: | "Major subject: Nutrition". Vita. |
| Physical Description: | x, 95 leaves : illustrations ; 28 cm. Also available online. Issued also on microfiche from Lange Micrographics. |
| Bibliography: | Includes bibliographical references. |