Ovarian nitric oxide synthase gene expression during peripubertal development /

(p<0.01) and LH (p<0.001). The INOS protein levels

Bibliographic Details
Main Author: Jones, Benjamin James
Format: Thesis eBook
Language:English
Published: [Place of publication not identified] : [publisher not identified] ; 1997.
Subjects:
Online Access:Link to OAKTrust copy
Description
Summary:(p<0.01) and LH (p<0.001). The INOS protein levels
accompanied by preovulatory increases in serum estradiol
analysis revealed both INOS and CNOS protein expression in
associated with declining (p<0.05) serum levels of estradiol.
before and after first ovulation; hence, suggesting a role
both forms of NOS were expressed in the ovary and that the
both the inducible (i) and constitutive (c) forms of NOS and
change during the peripubertal period. Specifically, there
donors in vitro, suggests that NO may be involved in ovarian
dramatically increased again to peak on the morning of first
during the early proestrous phase of development. By the
during the late proestrous phase (p < 0. 05) which was
each phase of puberty with only INOS showing a significant
estrous and diestrous phases. Furthermore, Western blot
estrus (p<0.001), i.e. a few hours after ovulation, an event
examined in the present study whether the ovary expresses
exogenously induced. Importantly, when compared with
for NO in the ovary during pubertal maturation and
function. However , neither the presence of NOS mRNA nor the
functions, some of which are associated with reproduction.
furthermore, providing compelling evidence at the gene level
in the expression of ovarian INOS MRNA and protein both
INOS MRNA transcripts were expressed without being
interest in ovarian function at the time of puberty, we
isoforms of the enzyme nitric oxide synthase. (NOS) and is
juvenile levels, INOS, but not CNOS, MRNA increased (p<0.01)
known to be involved in mediating several biological
late proestrous phase, the levels of INOS MRNA declined
markedly (p<0.001) and remained low throughout both the first
Much attention has been given to discerning the role of the
Nitric oxide (NO) is generated from L-arginine by different
NOINOS system regarding hypothalamic-pituitary hormone
phases of peripubertal development. Results indicate that
physiological role in ovarian function.
postovulatory events have been reported. Because of our
potential change in NOS gene expression relating to pre- and
presence or potential functions in the ovary- Recent data,
secretions with less attention devoted to discerning its
supporting the hypothesis that the NOINOS system plays a
These data are the first to demonstrate developmental changes
using NOS inhibitors both in vivo and in vitro, as well as NO
was an initial increase in the expression of INOS protein
whether either form undergoes changes in the ovary during the
Item Description:"Major subject: Veterinary Anatomy".
Vita.
Physical Description:viii, 38 leaves : illustrations ; 28 cm.
Also available online.
Issued also on microfiche from Lange Micrographics.
Bibliography:Includes bibliographical references.