http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
development of a rapid assay for patulin
also known to produce this mycotoxin. Patulin is most
and stable, thus facilitating its use in the prescreening of
anthroylnitrile. The derivative is added to the minicolumn
apples, grapes, and corn. It is produced primarily by
assay was found to be accurate and rapid. Also, the patulin
Briefly, the sample is extracted with ethyl acetate and
commonly found in apple products where its presence may
concentrating column). Patulin is then partitioned by
constitute a human health risk. At present, the best method
contaminated apple juice and products. A field-practical
detection for this assay was determined to be 50 ppb. This
detector and viewed under longwave (UV) light. The limit of
detector were shown to be chemically stable, requiring no
for control of patulin is the detection and diversion of
glass n-minicolumn at the interface of a layer of packed
level of 50 ppb or greater, can be easily detected as the
method for the chemiselective immobilization and detection of
neutral sand and narrow band silica gel. Patulin, at the
passed through a silica gel cartridge (or silica gel
patulin has been developed in our lab. In this new method,
Patulin is a mycotoxin found in many commodities including
Penicillium expansum and P. patulum although many other
polarity and eluted from the cartridge with acetonitrile.
presence of a bright yellow fluorescent band with this assay.
special treatment for storage up to 20 weeks. In summary,
species of Penicillium, Aspergillus, and Byssochlamys are
the assay was shown to be rapid, practical, easy to perform,
the derivative of patulin is selectively adsorbed in a small
The eluant (containing patulin) is derived with 9-
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