The metabolism of butyrate, glucose and glutamine in colonic epithelial cell lines /
a reduced NAD/NADH redox environment. Acutely, increased
| Main Author: | |
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| Format: | Thesis eBook |
| Language: | English |
| Published: |
[Place of publication not identified] :
[publisher not identified] ;
1997.
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| Subjects: | |
| Online Access: | Link to OAKTrust copy |
| Summary: | a reduced NAD/NADH redox environment. Acutely, increased all three colonic cell lines. The rate of butyrate oxidation and fate of metabolic substrates normally obtained either and glutamine. The cell line FRC/TEX/CLD, CL4D, provided by are used to examine the effects of butyrate (0, 0. 1, 1, 3 Butyrate and glutamine are the major respiratory fuels for butyrate, [6-14C] glucose, and [14C] glutamine. In addition, butyrate, or from metabolic substrates derived from the blood circulation to the colonic mucosa, i.e. glucose and colon, and two tumorigenic daughter cell lines, RAS and SRC, Dr. Summerhayes (Harvard University), derived from fetal rat effect, there are increased rates of glucose oxidation for formation from glucose, and the rate of macromolecular from colonic bacteria, i.e. short chain fatty acids such as Given that glutamine no longer provides the anaeploric glutamine. These experiments determined if butyrate, being growth inhibitory in colon cancer cells, also interferes with In order to delay the onset and advancement of cancer, this inhibiting the rate of glutamine oxidation. Glycolysis is inhibition of glutamine, and lack of ATP production. lactate production. Both acutely and chronically, butyrate measured. Butyrate suppresses growth, proliferation, DNA and mM) on the rates of oxidation and lipogenesis from [1-14 C] more TCA cycle intermediates. The high rate of lactate occurs in a dose dependent manner, while chronically proceeds through the TCA cycle at very high rates, producing proceeds through the TCA cycle is significantly suppressed. production could perhaps compensate for the chronic protein synthesis in transformed colonic cells; however, rates of glutamine proceeds through the TCA cycle to provide reduced NAD/NADH environment, the rate that glutamine research focused on inspecting the utilization, interaction, synthesis from [14C] glutamine and [3H] leucine are TCA cycle intermediates. However, chronically, due to the the addition of butyrate increasing glucose oxidation and the major pathway accounting for glucose disappearance, with the metabolism of other metabolic substrates, such as glucose the rate of ketogenesis from butyrate, the rate of lactate these effects are not observed in normal colonic cells. |
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| Item Description: | "Major subject: Nutrition". Vita. |
| Physical Description: | viii, 58 leaves : illustrations ; 28 cm. Also available online. Issued also on microfiche from Lange Micrographics. |
| Bibliography: | Includes bibliographical references: pages 50-57. |