rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Development of a map-based cloning system in Sorghum bicolor
    sub title: 1) isolation of megabase-size DNA and construction of a bacterial artificial chromosome library and, 2) genetic and physical mapping of the 5S rDNA locus
  name:
    name part: Woo, Sung-Sick
    name part: 1963-
    role:
      role term: creator
  type of resource: text
  genre: bibliography
  origin info:
    place:
      place term: xx
    date issued: 1996
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: print
    extent: xi, 202 leaves : illustrations ; 28 cm.
  abstract: A method was developed for the isolation of megabase-size DNA
  abstract: from Sorghum bicolor. The DNA prepared by this method was
  abstract: larger than 1 Mb in size and readily digestible with
  abstract: restriction enzymes. The DNA was shown to be suitable for
  abstract: physical mapping, and was successfully used for the
  abstract: construction of BAC and YAC libraries. The results
  abstract: demonstrate that this method will be useful for isolation of
  abstract: such DNA from sorghum and other closely related grasses such
  abstract: as sugarcane. The BAC system was tested in plants by
  abstract: constructing an ordered 13,440 clone sorghum BAC library.
  abstract: The library has an average insert size of 157 kb. Sorghum
  abstract: inserts of up to 315 kb were isolated and shown to be stable
  abstract: when grown for over 100 generations. No chimeric clones were
  abstract: detected as determined by fluorescence in situ hybridization.
  abstract: The library was screened with twelve sorghum and maize probes
  abstract: and all but one sorghum probe hybridized to at least one BAC
  abstract: clone. These results demonstrate that this BAC library will
  abstract: be useful for several physical mapping and map-based cloning
  abstract: applications not only in sorghum but other related cereal
  abstract: genomes. The sorghum 5S rRNA gene was investigated to study
  abstract: the molecular organization of the 5S RNA clusters using the
  abstract: method for isolation of megabase-size DNA from sorghum and
  abstract: the sorghum BAC library. Two size classes of 5S rDNA repeats
  abstract: were isolated from sBAC115Jl4, and one size class of 5S rDNA
  abstract: repeats was isolated from sBAC120A6. A large segment (118
  abstract: bp) is tandemly duplicated in the long 5S rDNA repeats. The
  abstract: 5S rDNA locus and two BACs were co-localized by FISH at one
  abstract: major locus on a medium size chromosome. The subtelomeric
  abstract: location of the 5S rDNA locus was confirmed by RFLP mapping
  abstract: of sequences immediately adjacent to the 5S rDNA locus. The
  abstract: results from the analysis of two BACs, PFGE analysis of
  abstract: several enzyme digests, and two different ladder type
  abstract: hybridization patterns of 5S rDNA repeats in genomic DNA
  abstract: suggested that two arrays of repeats, having evidently
  abstract: different characters, co-exist in the same 5S rDNA locus of
  abstract: sorghum.
  note: by Sung-Sick Woo.
  note: Vita.
  note: "Major Subject: Genetics".
  note: In title, numerals are used.
  note: Issued also on microfiche from University Microfilms Inc.
  note: Includes bibliographical references: pages 179-201.
  note: Ph. D. Texas A&M University 1996.
  subject:
    topic: Major genetics
  identifier: 97-18480 UMI
  identifier: http://proxy.library.tamu.edu/login
  related item:
    location:
      url: http://proxy.library.tamu.edu/login
  record info:
    record content source: TXA
    record creation date: 970813
    record change date: 20190325093328.0
    record identifier: in00001379170