Separation efficiency and mobility matching in capillary electrophoresis /
In capillary electrophoresis the addition of a sample band
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| Format: | Thesis Book |
| Language: | English |
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[Place of publication not identified] :
[publisher not identified] ;
1996.
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| Online Access: | http://proxy.library.tamu.edu/login?url=http://proquest.umi.com/pqdweb?did=739668091&sid=1&Fmt=2&clientId=2945&RQT=309&VName=PQD |
| Summary: | In capillary electrophoresis the addition of a sample band disrupts the local electric field strength in the capillary. The electric field disruption distorts the shape of the analyte peaks and manifests itself in fronting or tailing profiles. This phenomenon, electromigration dispersion, is detrimental in the separation of closely migrating analytes. Theoretical calculations have shown that electromigration dispersion may be minimized when the conductance of the sample band is matched to the conductance of the background electrolyte (BGE). The conductance matching may be best achieved when the mobility of the analyte is close to the mobility of the BGE co-ion. A series of cations and anions have been synthesized and characterized for use as mobility matching co-ions in capillary electrophoresis and their mobilities span the 5 to 40x 10- 5cm2/Vs range. The polyethylene glycol mono methyl ether based mobility matching reagents do not interfere with the chiral recognition characteristics of p-cyclodextrin. The use of multiple co-ion BGEs has been modeled using the Dose- Guiochon simulation program of electrophoresis. The computer simulations indicated that the closer the co-ion mobilities were in the multiple co-ion BGEs the better the efficiency. Multiple component strong acids and bases were synthesized and used in the electrophoretic separation of closely migrating analytes. Good agreement was observed between the simulated peak profiles and those observed using the multiple co-ion BGEs. Utilizing the mobility matching background electrolyte concept, the pH buffering action of the BGE may be separated from the mobility matching function, thus allowing the selection of both the appropriate BGE pH and the appropriate co-ion whose mobility can be matched to the analyte of interest. Furthermore, the multiple co-ion BGEs provided efficiency improvement over a mobility range as large as 10x 10-5 cm2/Vs. |
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| Item Description: | Vita. "Major Subject: Chemistry". |
| Physical Description: | xiv, 122 leaves : illustrations ; 28 cm. Issued also on microfiche from University Microfilms Inc. |
| Bibliography: | Includes bibliographical references: pages 118-120. |