Determining the evolutionary relationship of human chromosome 17 and bovine chromosome 19 by comparative gene mapping /
Comparative gene mapping may be accomplished by a variety of
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| Format: | Thesis Book |
| Language: | English |
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[Place of publication not identified] :
[publisher not identified] ;
1996.
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| Online Access: | http://proxy.library.tamu.edu/login?url=http://proquest.umi.com/pqdweb?did=739669311&sid=1&Fmt=2&clientId=2945&RQT=309&VName=PQD |
| Summary: | Comparative gene mapping may be accomplished by a variety of methods including synteny mapping and linkage mapping. The former is generally achieved by somatic cell mapping of homologous type I loci in different species to identify evolutionarily conserved syntenic segments, while the latter addresses gene order and linkage conservation within conserved syntenic segments. Somatic cell mapping provides little if any information about the order of loci within a conserved segment. A study of conservation of gene order within conserved segments is necessary to generate refined comparative maps capable of providing information on internal rearrangements in chromosome evolution. This dissertation is a study of the evolutionary relationship of human chromosome 17 (HSA17) and bovine chromosome 19 (BTA19) both in terms of synteny conservation and internal structural rearrangement. First, eight comparative anchor loci on HSA17 (CHRNB1, CRYB1, MYL4, MPO, NF1, P4HB, THRA1, and TP53) were assigned to BTA19 using cattle X rodent somatic hybrid cell lines. This completed the bovine synteny mapping of human chromosome 17 comparative anchor loci, all of which have been mapped to bovine chromosome 19 and mouse chromosome 11 (MMU11). Second, conservation of gene order within the conserved segment represented by HSA17, BTA19, and MMUll was explored by different strategies including linkage studies in an international bovine reference family (IBRF) panel and in a bovine interspecific hybrid backcross (IHB) panel, and by whole genome radiation hybrid (WG-RH) mapping. Eight comparative anchor loci on HSA17 and five microsatellite markers were included in the BTA19 linkage map by the IBH method. Seven HSA17 comparative anchor loci and nine microsatellite markers were placed on the WG-RH map of BTA19. The orders obtained by the three mapping methods are in agreement. This study expands our knowledge of physical, linkage and comparative maps of BTA19 and introduces two new strategies, IHB and WG-RH mapping for cattle gene mapping. These new methods have the advantage of being more efficient than traditional bovine linkage studies for mapping type I loci and their successful application in cattle will greatly benefit the comparative mapping of the cattle genome. |
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| Item Description: | Vita. "Major Subject: Genetics". In title, numerals are used. |
| Physical Description: | xi, 105 leaves : illustrations ; 28 cm. Issued also on microfiche from University Microfilms Inc. |
| Bibliography: | Includes bibliographical references: pages 80-95. |