Effects of bursal anti-steroidogenic peptide (BASP) on chicken lymphocyte and anterior pituitary cell function /
Previously, our laboratory has reported the isolation and partial characterization of bursal anti-steroidogenic peptide (BASP) from the chicken bursa of Fabricius. This molecule has demonstrated potent inhibitory activities on chicken ovarian granuloma and adrenocortical cell steroid biosynthesis....
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| Format: | Thesis Book |
| Language: | English |
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[Place of publication not identified] :
[publisher not identified] ;
1997.
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| Online Access: | http://proxy.library.tamu.edu/login?url=http://proquest.umi.com/pqdweb?did=739891221&sid=1&Fmt=2&clientId=2945&RQT=309&VName=PQD |
| Summary: | Previously, our laboratory has reported the isolation and partial characterization of bursal anti-steroidogenic peptide (BASP) from the chicken bursa of Fabricius. This molecule has demonstrated potent inhibitory activities on chicken ovarian granuloma and adrenocortical cell steroid biosynthesis. In the present manuscript, we report the effects of BASP on selected parameters of lymphocyte function in isolated lymphocyte pools derived from chicken, rat, and human subjects. These investigations have shown BASP to be capable of attenuating mitogen-induced DNA synthesis in neonatal chick bursal lymphocytes. Similar experiments have demonstrated BASP to be efficacious in reducing mitogen-stimulated DNA synthesis in peripheral blood lymphocytes derived from chicken, rat, and human sources. To compare these bioactivities of BASP with activities of molecules known to affect B-lymphocyte maturation, the effects of BASP and human recombinant insulin-like growth factor-1 (IGF-1) were compared alone and in combination. These experiments demonstrated the ability of IGF-1 to attenuate mitogen-induced DNA synthesis in neonatal chick bursal-lymphocytes. Additionally, a small additive effect in the reduction in neonatal chick DNA synthesis was observed by combining sub-optimal concentrations of BASP with IGF-1. Other sets of experiments in the present manuscript evaluated the ability of BASP to affect chicken embryonic anterior pituitary cell secretion of growth hormone (GH) and prolactin (PRL). These experiments demonstrated a standard secretogogue activity of BASP in eliciting the secretion of both GH and PRL, when evaluated independently in a reverse hemolytic plaque assay (RHPA). Data derived from the previous sets of experiments in the present manuscript suggest the potential for BASP to affect lymphocyte and anterior pituitary function in the chicken. |
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| Item Description: | Vita. "Major Subject: Veterinary Microbiology". |
| Physical Description: | x, 114 leaves : illustrations ; 28 cm. Issued also on microfiche from University Microfilms Inc. |
| Bibliography: | Includes bibliographical references: pages 102-113. |