Individual-variation and inheritance of aphidicolin-induced chromosomal fragile sites in Peromyscus maniculatus /
This research examined the individual-variation and inheritance of aphidicolin-induced chromosomal fragile sites of deer mice Peromyscus maniculatus. The FSM statistical methodology allowed the identification of fragile sites in single individuals. Six deer mice from a natural population were used...
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| Format: | Thesis Book |
| Language: | English |
| Published: |
[Place of publication not identified] :
[publisher not identified] ;
1996.
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| Online Access: | http://proxy.library.tamu.edu/login?url=http://proquest.umi.com/pqdweb?did=739640021&sid=1&Fmt=2&clientId=2945&RQT=309&VName=PQD |
| Summary: | This research examined the individual-variation and inheritance of aphidicolin-induced chromosomal fragile sites of deer mice Peromyscus maniculatus. The FSM statistical methodology allowed the identification of fragile sites in single individuals. Six deer mice from a natural population were used to establish three breeding pairs. Individual variation was extensive with the number of fragile sites ranging from 11 to 45 in the parental individuals. Each fragile site was considered as a separate locus and, using a probability-based procedure, genotypes were assigned as presence/absence characters in the three families. Allelic frequencies were determined and each locus was tested for conformation to expected proportions assuming codominant Mendelian inheritance. The results of this analysis suggest that genetic factors are instrumental in the expression of aphidicolin-induced fragile sites. Chromosomal morphology conformed to the pattern previously described for Peromyscus. Chromosomes polymorphic for pericentric inversions included 6 and 15. Heterochromatin polymorphisms were observed for chromosomes 8, 12, 16, and 17. The segregation patterns of these chromosomal polymorphisms were found to conform to expected Mendelian proportions with the exception of chromosome 6. Additionally, fragile sites were present at each of the inversion breakpoints suggesting that fragile sites may represent sites prone to rearrangements involved in chromosomal evolution. |
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| Item Description: | Vita. "Major Subject: Genetics". |
| Physical Description: | viii, 82 leaves : illustrations ; 28 cm. Issued also on microfiche from University Microfilms Inc. |
| Bibliography: | Includes bibliographical references: pages 53-68. |