Isolation and characterization of PhoP homolog in Brucella abortus /
abortus k Zapll genomic library, which contained the intact
| Main Author: | |
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| Format: | Thesis eBook |
| Language: | English |
| Published: |
[Place of publication not identified] :
[publisher not identified] ;
1997.
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| Subjects: | |
| Online Access: | Link to OAKTrust copy |
| Summary: | abortus k Zapll genomic library, which contained the intact acid sequences shared by S. typhimurium phop and E. coli and wild type strains in peptide sensitivity and hydrogen deduced amino acid sequence of tcrp exhibited 41% homology to defensin and acid. In this study, a phop-like gene designated as tcrp was isolated from B. abortus. Following discussed. due to 0-antigen defect. The tcrp mutant also showed a experiments for further characterization of this gene were fold) sensitivity to complement mediated lysis, which was not fragment using PCR degerate primers based on homologous amino members of two-component regulatory proteins. A B. abortus peroxide killing tests. Based on the preliminary phenotypic phob, a larger DNA fragment (3355 bp) was isolated from B. PhoP/PhoQ two-component regulatory system which controls putative functions of the tcrp gene. The results from these S. typhimurium PhoP and also showed general features of the Salmonella phoP::TnlO mutants are highly sensitive to serum, significant differences were found between the tcrp mutants slightly increased acid sensitivity (1.5 fold). No strain carrying tcrp mutation was constructed for testing the tcrp gene and its upstream and downstream sequences. The test showed that the tcrp mutants exhibited increased (10 tests, the putative functions of tcrp gene and the the initial amplification of a 300 base pair phop-like gene typhimurium has been detected in several other species. virulence and intramacrophage survival of Salmonella |
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| Item Description: | "Major subject: Veterinary Microbiology". Vita. |
| Physical Description: | xi, 76 leaves : illustrations ; 28 cm. Also available online. Issued also on microfiche from Lange Micrographics. |
| Bibliography: | Includes bibliographical references. |