Isolation, sequencing, and the genomic organization of the reduced folate carrier gene in the murine system /
administration of periconceptional supplemental folic acid.
| Main Author: | |
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| Format: | Thesis eBook |
| Language: | English |
| Published: |
[Place of publication not identified] :
[publisher not identified] ;
1996.
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| Subjects: | |
| Online Access: | Link to OAKTrust copy |
| Summary: | administration of periconceptional supplemental folic acid. an average rate in the U.S. of 1 per 1000 live-births with and seven introns. The gene forms at least four splicing as an extension of exon 3a. Future studies will be initiated being investigated. The reduced folate carrier (RFC) between altered RFC function, folate supplementation, and the bifida and anencephaly. Neural tube defects (NTDS) occur at carbon transfers in critical biochemical pathways leading to compromise DNA synthesis and cellular proliferation, constructed. Utilizing this model system, correlations Defective closure of the neural tube during embryogenesis delivers 5-methyltetrahydrofolate from the folate receptors deriving sequencing primers from the known CDNA sequence of DNA synthesis. Alterations in the folate pathway could end, the genetic regulation of folate internalization is expression will be localized and a knock-out mouse for subcloning. An approximately 1OKb positive clone was gene in the mouse. Screening of a murine ES cell genomic genomic sequence was derived. The gene contains eight exons in relation to the folate binding proteins. The RFC variant internalization, we isolated and partially sequenced the RFC Intracellular folates are believed to be responsible for the library produced eight positive clones; three were selected occurrence of NTDs may be elucidated. potentially disrupting normal developmental events. To this rates. Several investigators have shown a marked decrease in reduction in NTDs due to their role in facilitating single- results in serious and common birth defects such as spina selected ethnic groups having higher or lower prevalence subcloned into pbluescript and has been divided into smaller subclones. Utilizing each subc[one independently, and the gene, followed by chromosome walking, 5.6Kb of the the occurrence and recurrence rates of NTDs with the the relationship between NTDs and aberrant folate to determine the tissue specific expression of the RFC gene to the cytoplasm of the cell. As a first step in defining variants with alternate exons located 5'of exons 1 and 5, and |
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| Item Description: | "Major subject: Genetics". Vita. |
| Physical Description: | vii, 78 leaves : illustrations ; 28 cm. Also available online. Issued also on microfiche from Lange Micrographics. |
| Bibliography: | Includes bibliographical references: pages 64-77. |