Isolation and characterization of a cDNA for the beta subunit of chicken Thyroid-Stimulating Hormone /
after the detection of GH in circulation on day 19 of
| Main Author: | |
|---|---|
| Format: | Thesis eBook |
| Language: | English |
| Published: |
[Place of publication not identified] :
[publisher not identified] ;
1996.
|
| Subjects: | |
| Online Access: | Link to OAKTrust copy |
| Summary: | after the detection of GH in circulation on day 19 of and growth hormone (GH) during late embyonic and early post- bases). Once a probe was developed, TSHP MRNA levels were been isolated and chain reaction (PCR) was implemented and a homologous CDNA day 1. T3 levels increased significantly on post-hatch day I embryonic development. The regulation of TSHP gene expression around the time of hatch. Comparison of the CDNA expression by T3 and thyrotropin releasing hormone (TRH) is expression on embryonic day 19 and post-hatch day 1. In these followed that pattern, with a sharp increase on post-hatch for chicken TSHP and use this CDNA to characterize TSHP gene frog. However, TSHO has not been isolated from any avian frog) identified three highly conserved regions. Polymerase had no effect. hatch ages of chickens. In these experiments it was shown hybridized to an RNA species of approximately 500 bases in measured along with thyroxine (T,), triiodothyronine (T 3), MRNA levels, while T3 had no significant effects. However, on day I T 3 decreased levels of TSHP MRNA, while TRH other tissues. This length corresponds with the length of pituitary RNA samples alone; no signal was detected in the sequence was produced. Tissue specificity of the CDNA probe sequenced in many species including several mammals and the sequences for TSHP from five species (four mammals and the species. The objective of this study was to isolate a CDNA studies we found that on embryonic day 19 TRH stimulated TSHP that TSHO MRNA peaked on embryonic day 19 and T4 levels The beta subunit of Thyroid Stimulating Hormone (TSHP) has TSHP MRNA in other species (that range from 450 to 575 was analysed by Northern blot. The "P-labeled probe was to test the effect of TRH or T3 on chicken TSHP gene well documented in mammals. The objective of the next study |
|---|---|
| Item Description: | "Major subject: Poultry Science". Vita. |
| Physical Description: | viii, 63 leaves : illustrations ; 28 cm. Also available online. Issued also on microfiche from Lange Micrographics. |
| Bibliography: | Includes bibliographical references: pages 55-62. |