http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
xx, 521 pages : illustrations (some color) ; 24 cm.
1. Introduction -- Recombinanat gene expression protocols overview of experimental strategies for the expression of recombinant genes -- 2. Expression in E. coli -- Designing expression plasmid vectors in E. coli -- Design of bacterial hosts for Iac-based expressed vectors -- Bacteriophage gamma-based expression vectors -- Strategies for gene fusions -- Application of the E. coli trp promoter -- Expression of proteins in E. coli utilizing a dual promoter-based vector: pLACT7 -- Expression and secretion of proteins in E. coli -- Synthetic two-cistron expression system -- Expression of cDNA-encoded proteins by cell-free transcription and translation -- 3. Expression in yeast -- Yeast plasids -- Inducible expression cassettes in yeast: GAL4 -- Inducible expression cassettes in yeast: ADH2 -- Constitutive expression vectors: PGK -- 4. Viral expression systems -- Design and construction of recombinant vaccinia viruses -- A simplified method of screening for isolation of recombinant vaccinia virus -- Design of retroviral expression vectors -- Use of defective herpes-derived plasmid vectors -- 5. Expression in nonmammalian higher eukaryotes -- Use of baculovirus expression vectors -- Expression of exogenous genes in Xenopus oocytes, eggs, and embryos -- Analysis of heterologous gene expression in Xenopus blastomeres -- 6. Expression in mammalian cells -- Overview of vector design for mammalian gene expression -- Experimental strategies in efficient transfection of mammalian cells: calcium phosphate and DAAE-dextran -- Experimental strategies in efficient transfection of mammalian cells: electroporation -- Highly effective delivery of foreign DNA to atherent cells via polybrene/DMSO-assisted gene transfer -- Selection of transfected cells: magnetic affinity cell sorting -- Selection of transfected cells and coamplification of transfected genes -- Optimization of growth, viability, and specific productivity for expression of recombinant proteins in mammalian cells -- 7. Expression in transgenic organisms -- 8. Expression in Plants.
Includes bibliographical references and index.
Methods in molecular biology (Clifton, N.J.) ; v. 62