rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Recombinant gene expression protocols
  name:
    name part: Tuan, Rocky S.
  type of resource: text
  genre: bibliography
  origin info:
    place:
      place term: nju
    date issued: 1997
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: print
    extent: xx, 521 pages : illustrations (some color) ; 24 cm.
  table of contents: 1. Introduction -- Recombinanat gene expression protocols overview of experimental strategies for the expression of recombinant genes -- 2. Expression in E. coli -- Designing expression plasmid vectors in E. coli -- Design of bacterial hosts for Iac-based expressed vectors -- Bacteriophage gamma-based expression vectors -- Strategies for gene fusions -- Application of the E. coli trp promoter -- Expression of proteins in E. coli utilizing a dual promoter-based vector: pLACT7 -- Expression and secretion of proteins in E. coli -- Synthetic two-cistron expression system -- Expression of cDNA-encoded proteins by cell-free transcription and translation -- 3. Expression in yeast -- Yeast plasids -- Inducible expression cassettes in yeast: GAL4 -- Inducible expression cassettes in yeast: ADH2 -- Constitutive expression vectors: PGK -- 4. Viral expression systems -- Design and construction of recombinant vaccinia viruses -- A simplified method of screening for isolation of recombinant vaccinia virus -- Design of retroviral expression vectors -- Use of defective herpes-derived plasmid vectors -- 5. Expression in nonmammalian higher eukaryotes -- Use of baculovirus expression vectors -- Expression of exogenous genes in Xenopus oocytes, eggs, and embryos -- Analysis of heterologous gene expression in Xenopus blastomeres -- 6. Expression in mammalian cells -- Overview of vector design for mammalian gene expression -- Experimental strategies in efficient transfection of mammalian cells: calcium phosphate and DAAE-dextran -- Experimental strategies in efficient transfection of mammalian cells: electroporation -- Highly effective delivery of foreign DNA to atherent cells via polybrene/DMSO-assisted gene transfer -- Selection of transfected cells: magnetic affinity cell sorting -- Selection of transfected cells and coamplification of transfected genes -- Optimization of growth, viability, and specific productivity for expression of recombinant proteins in mammalian cells -- 7. Expr…
  note: edited by Rocky S. Tuan.
  note: Includes bibliographical references and index.
  subject:
    topic: Genetic recombination
    topic: Laboratory manuals
  subject:
    topic: Recombination, Genetic
    genre: laboratory manuals
  subject:
    topic: Gene Expression
    genre: laboratory manuals
  subject:
    topic: Genetic Engineering
    genre: laboratory manuals
  subject:
    topic: Genetic recombination
    topic: Laboratory manuals
  subject:
    topic: Acidos nucleicos
  subject:
    topic: Biologia molecular e macromolecular
  classification: QH443 .R36 1997
  classification: 572.8/77
  classification: W1 ME9616J v.62 1997
  classification: QH 443 R3108 1997
  related item:
    title info:
      title: Methods in molecular biology (Clifton, N.J.) ; v. 62
  identifier: 0896033333 (alk. paper)
  identifier: 0896034801 (hardcover)
  identifier: 96049600
  record info:
    record content source: DLC
    record creation date: 961107
    record change date: 20151006074045.0
    record identifier: in00001372073