Artful leadership : managing stakeholder problems in nonprofit arts organizations /
Absorption of selenate in the presence of sulfate 3-108) and index.
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| Format: | Book |
| Language: | English |
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Bloomington :
Indiana University Press,
[1996]
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| Series: | IU Center on Philanthropy series in governance.
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| Subjects: |
| Summary: | Absorption of selenate in the presence of sulfate 3-108) and index. followed the mechanism proposed by Kochian and Lucas with saturable and linear phases. High concentrations of sulfate in solution culture(5mM) affected b0th phases resulting in reduced absorption of selenate. A low concentration of sulfate (0.05mM) improved absorption of selenate at the root surfaces. OPA-derivatized amino acid separation on a HPLC column was evaluated as a means to separate and quantify cystine, selenocystine, methionine and selenomethionine. Selenomethionine and methionine were separated by this method, but OPA did not react with selenocystine. Oxidised selenocystine reacted with OPA, but the retention time was at void volume, and therefore was not useful for detection in plant extracts. Free methionine and selenomethionine concentrations were determined in Se treated, solution culture-grown plant extracts using this HPLC method. Astralagus bisulcatus, a selenium accumulator, did not accumulate selenomethionine. Astralagus lentiginosus and Melilotus indica, Both selenium non accumulators, contained detectable amounts of selenomethionine. Average methionine contents increased in shoots of Se treated plants compared to controls, but the increase was statistically significant only for A.lentiginosus and M.indica. Average concentrations of methionine decreased in roots of all Se treated plants compared to the controls, and the decrease was statistically significant for T.repens and M.indica. The HPLC method was useful to detect methionine and selenomethionine in M.indica plants that grew in Kesterson. |
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| Physical Description: | x, 116 pages : illustrations ; 24 cm. |
| ISBN: | 0253332346 (alk. paper) |