Development of a carotenoid shuttle vector for Lactobacillus /
acquisition was analyzed and verified by agarose gel
| Main Author: | |
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| Format: | Thesis eBook |
| Language: | English |
| Published: |
[Place of publication not identified] :
[publisher not identified] ;
1996.
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| Subjects: | |
| Online Access: | Link to OAKTrust copy |
| Summary: | acquisition was analyzed and verified by agarose gel and carotenoid biosynthesis after shuttling. A Lactobacillus biosynthesis pathway isolated from Erwinia uredovora, and carotenoid biosynthesis gene cluster between E. coli and L. casei, and evaluate the structural stability of the plasmid coli. This insert was ligated into linearized PCAR 1 6, a colonies due to synthesis of 0-carotene. Results indicate electrophoresis. Plasmid PCSVK I was then electroporated expresses 0-carotene synthesis in E. coli. The recombinant from plasmid pLP503, a shuttle vector for Lactobacillus and E functioning as a shuttle vector between E coli and L. casei. gel electrophoresis. Plasmid PCSVKI prepared from L. casei into L. casei and acquisition was analyzed and verified by its ability to express 0-carotene synthesis in E. coli while plasmid vector which contains a portion of the carotenoid replicon and an erythromycin resistance marker was prepared shuttle vector (PCSVKI) resulting from this ligation was that plasmid PCSVKI maintains its structural stability and The following studies describe the construction of a plasmid transformed into E. coli resulting in orange colonies; vector, using E. coli as a host, designed to shuttle a was retransformed into E coli, again resulting in orange |
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| Item Description: | "Major subject: Nutrition". Vita. |
| Physical Description: | viii, 44leaves : illustrations ; 28 cm. Also available online. Issued also on microfiche from Lange Micrographics. |
| Bibliography: | Includes bibliographical references: pages 37-43. |