Colonization, hybridization and insecticide supceptibility studies of Culex salinarius CoQ.(Diptera: Culicidae) /
Techniques for colonization of Culex salinarius were developed and used to establish laboratory colonies of Texas and New Jersey strains of this species. This was accomplished in an environmental chamber set at 20 'C, 80% RH and a 10:14 L:D photoperiod having ca. 1-h of crepuscular period. T...
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| Format: | Thesis Book |
| Language: | English |
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[Place of publication not identified] :
[publisher not identified] ;
1996.
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| Online Access: | http://proxy.library.tamu.edu/login?url=http://proquest.umi.com/pqdweb?did=739668291&sid=1&Fmt=2&clientId=2945&RQT=309&VName=PQD |
| Summary: | Techniques for colonization of Culex salinarius were developed and used to establish laboratory colonies of Texas and New Jersey strains of this species. This was accomplished in an environmental chamber set at 20 'C, 80% RH and a 10:14 L:D photoperiod having ca. 1-h of crepuscular period. The adults were allowed to mate naturally in 60 x 60 x 60 cm cages. Larvae were fed daily with a mixture of 1:1:1 lab chow, brewer's yeast and lactalbumin suspended in deionized water. Dry Bermuda grass, Cynodon dactvlon L., was added in the larval rearing trays. Sugar water and live chickens were provided as the carbohydrate source and blood meal source, respectively. Larval development times for Texas and New Jersey strains of Cx. salinarius were 9-12 days and 9-15 days, respectively, while pupal development time was 2 days for both strains. The average larval survival rate and survival rate from 1st instar larvae to the adult for the Texas strain were 60% and 59%, respectively, with the female:male adult sex ratio being 1:1.4. The average larval survival rate and survival rate from 1st instar larvae to the adult for the New Jersey strain were 72.3% and 72%, respectively, with the female:male adult sex ratio being 1:1.1. Cross-mating experiments were performed using the Texas and New Jersey Cx. salinarius colonies to determine whether or not Cx. salinarius might be a species complex. Hatching rates, larval and pupal survival rates and survival from last instar larvae to adults of parental crosses, reciprocal crosses, F, of reciprocal crosses and backcrosses indicated that no reproductive isolation existed between laboratory colonies of Texas and New Jersey. It is concluded that the Texas and New Jersey stains of Cx. salinarius belong to the same species. Insecticide susceptibility assessments of Texas and New Jersey strains of Cx. salinarius were performed using the insecticide-coated vial technique. Adult females of the New Jersey strain are ca. 9 times more tolerant to malathion than are those of the Texas strain, with the LC50 rate being 0.701 mg malathion/vial and 0.076 mg malathion/vial for each strain, respectively. Females of both strains are similar in their susceptibility to naled, chlorpyrifos, resmethrin and permethrin. |
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| Item Description: | Vita. "Major Subject: Entomology". |
| Physical Description: | xiv, 163 leaves : illustrations ; 28 cm. Issued also on microfiche from University Microfilms Inc. |
| Bibliography: | Includes bibliographical references: pages 154-162. |