RAPD polymorphisms in diploid plums : genetic relationships and genetic linkage maps /
RAPD analysis was used to analyze genetic diversity and relationships of six diploid plum species from two sections and four cultivated Japanese-type plum gene pools; and to assess species composition of interspecific founding clones of cultivated Japanese-type plums. Ten primers amplifying 168 RAP...
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| Format: | Thesis Book |
| Language: | English |
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[Place of publication not identified] :
[publisher not identified] ;
1996.
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| Subjects: | |
| Online Access: | http://proxy.library.tamu.edu/login?url=http://proquest.umi.com/pqdweb?did=739640091&sid=1&Fmt=2&clientId=2945&RQT=309&VName=PQD |
| Summary: | RAPD analysis was used to analyze genetic diversity and relationships of six diploid plum species from two sections and four cultivated Japanese-type plum gene pools; and to assess species composition of interspecific founding clones of cultivated Japanese-type plums. Ten primers amplifying 168 RAPD markers were used. One-third of the RAPD markers were shared by species between the sections; while, two- thirds were shared by species within the sections. The Nei and Li similarity coefficients (NL) were comparable indicating that genetic background of plum species within the section was twice as similar as those between the sections due to common ancestors. The UPGMA cluster analysis on NL produced a dendrogrom that agreed with taxonomic classification. Cultivated plums from the Florida gene pool were unique and clustered together; while, the other gene pools clustered with each other depending on their genetic background. Cultivated plums with P. angustifolia background showed the least similarity with other plums. Genetic diversity of the southeastern US was the most followed by the South African, the California, and the Florida gene pools. The most genetic similarity between gene pools was between the California and the South African, and the least was between the southeastern US and the Florida gene pools. Four putative species contributed to founding clones. P. salicina was predominant genetic background with at least a one-third contribution- while, P. americana contributed the least at one-tenth. P. simonii and P. cerasifera were comparable at one-fourth. Pseudo-testcross mapping strategy using RAPD and isozyme markers was used to construct genetic linkage maps from 81 hybrids of 'Robusto' x 'Laroda'. Fifty-seven of 240 primers screened generated 172 segregating RAPD markers. Four isozyme systems were analyzed. Forty-two RAPD and one isozyme markers showed segregation distortion. One hundred- twenty markers were mapped for 'Robusto' resulting in eight linkage groups and six unlinked markers. Fifty-seven markers were mapped for 'Laroda' resulting in 11 linkage groups and five unlinked markers. These maps cover approximately 78% and 46% of its genome, respectively. The error of RAPD analysis due to independent DNA extraction was 2.5% and that due to inconsistency between RAPD assay was 1%. |
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| Item Description: | Vita. "Major Subject: Plant Breeding". |
| Physical Description: | x, 128 leaves : illustrations ; 28 cm. Issued also on microfiche from University Microfilms Inc. |
| Bibliography: | Includes bibliographical references: pages 91-99. |