Stress proteins and disease susceptibility /
As a response to adverse stimuli in their local environment, cells from all organisms produce a family of proteins referred to as heat shock or stress proteins. As a group, stress proteins are thought to protect the cell from various environmental insults and promote recovery of metabolic pathways...
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| Format: | Thesis Book |
| Language: | English |
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[Place of publication not identified] :
[publisher not identified] ;
1996.
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| Online Access: | http://proxy.library.tamu.edu/login?url=http://proquest.umi.com/pqdweb?did=739363571&sid=1&Fmt=2&clientId=2945&RQT=309&VName=PQD |
| Summary: | As a response to adverse stimuli in their local environment, cells from all organisms produce a family of proteins referred to as heat shock or stress proteins. As a group, stress proteins are thought to protect the cell from various environmental insults and promote recovery of metabolic pathways disturbed as a result of a stressful event. Stress proteins are also expressed in unstressed cells and participate in a number of cellular functions. In this dissertation stress proteins were examined in three different models to determine if stress protein expression coincided with stress and altered susceptibility to disease. In the first model, cattle were classified as either thermotolerant or thermosensitive based on phenotypic and genotypic breed characteristics. Using peripheral blood mononuclear cells of thermotolerant or thermosensitive animals, the relationship between stress protein expression and functional capacity of cells following heat stress was assessed. No statistical differences were noted between the two groups. In the second model, macrophages were isolated from cows classified as Brucella abortus resistant or susceptible. Experiments demonstrated that stress proteins did not play a role in resistance or susceptibility to Brucella abortus in bovine macrophages. In the third system, a caprine model for bovine respiratory disease (shipping fever) was established to investigate stress, stress proteins, and disease susceptibility. In this study, goats underwent a series of vaccinations with Pasteurel.la haemolytica followed by challenge with live Pasteurella. Throughout the study classical indicators of stress (temperature, ACTH, and cortisol) and cellular indicators of stress (stress proteins) were evaluated to determine if a relationship existed between stress, stress proteins, and susceptibility to Pasteurella infection. Experiments did not demonstrate a relationship between stress protein expression and temperature, ACTH, and cortisol or alterations in T cell populations. Stress can alter immune function and there is an association between stress and an increased susceptibility to disease. Identification of markers that correlate with increased susceptibility would be of great value in human and animal health. Studies presented here have investigated selected heat shock proteins as candidate markers of disease susceptibility. No correlations between expression of heat shock proteins and disease susceptibility were found. |
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| Item Description: | Vita. "Major Subject: Veterinary Physiology". |
| Physical Description: | ix, 152 leaves : illustrations ; 28 cm. Issued also on microfiche from University Microfilms Inc. |
| Bibliography: | Includes bibliographical references: pages 124-151. |