Differentiation of modern sorghum bicolor (L.) moench cultivars using fourier transform infrared spectroscopy (FTIR) and gas-liquid chromatography (GLC) /
This dissertation describes the use of Fourier transform
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| Format: | Thesis Book |
| Language: | English |
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[Place of publication not identified] :
[publisher not identified] ;
1996.
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| Online Access: | http://proxy.library.tamu.edu/login?url=http://proquest.umi.com/pqdweb?did=739363661&sid=1&Fmt=2&clientId=2945&RQT=309&VName=PQD |
| Summary: | This dissertation describes the use of Fourier transform infrared spectroscopy (FTIR) and gas liquid chromatography (GLC) to attempt to discriminate between wild and cultivated grains of modern Sorghum bicolor (L) Moench. I used mutlivariate statistical methods for analyzing the chemical compositional data of the modern sorghum grains. The results were obtained by extraction of lipids from a single modern sorghum grain using organic solvents. Spectral analysis on the extracted lipid material was performed using Fourier transform infrared spectroscopy. Thin-layer chromatography was used to separate the lipid material into neutral and polar components. Identification and quantification of the fatty acids in the neutral lipid component was achieved using gas liquid chromatography. The principal findings of this study are: (1)No differentiation between cultivated and wild seeds of Sorghum bicolor using FTIR spectra analysis was observed due to the similarity within the fingerprint region (1200 cm-' and 600 cm-'). (2)Spectra for the cultivars of both cultivated and wild seeds suggest that the lipid extraction contains a non-aromatic ester. (3)Seven fatty acids were identified and quantified using GLC. Discrimination between modern cultivated and spontaneous races within Sorghum bicolor (L.) Moench was established with two fatty acids, linoleic acid (Cl8:2) and palmitic acid (Cl6:0) identified in our lipid extract from the sorghum grains. We are confident that this analytical method could be used on modern sorghum grains to further distinguish chemically between cultivated and spontaneous races within Sorghum bicolor (L) Moench. (4) Further work is needed to establish whether GLC would be -useful for classifying archaeological seeds as cultivated or spontaneous. It needs to be determined whether there is a significant decrease in the unsaturated fatty acid concentrations of the neutral lipids in plants. Furthermore, whether degradation of the fatty acids over time would change the relative concentrations of the fatty acids needs investigation. |
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| Item Description: | Vita. "Major Subject: Chemistry". |
| Physical Description: | xi, 98 leaves : illustrations ; 28 cm. Issued also on microfiche from University Microfilms Inc. |
| Bibliography: | Includes bibliographical references : pages 90-97. |