A postsource decay study of bradykinin by Matrix-assisted laser desorption ionization mass spectrometry /
[M+H]+ ions, that occur in the field-free region of the
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| Format: | Thesis eBook |
| Language: | English |
| Published: |
[Place of publication not identified] :
[publisher not identified] ;
1996.
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| Subjects: | |
| Online Access: | Link to OAKTrust copy |
| Summary: | [M+H]+ ions, that occur in the field-free region of the addition of water to methanol increases the abundance of PSD at positions 3 and 7 of bradykinin affects the abundance of bradykinin (hyp stands for 4-hydroxyproline), using MALDI-TOF bradykinin and analogues, [D-Phe7]-bradykinin and hyp3- bradykinin fragmentation is also shown here. It appears that focuses on the fragmentation reactions of the hormone fragment ions and the use of trifluoroacetic acid (TFA) in Fragmentation reactions of the protonated analyte molecule, ions in the PSD spectrum of [D-Phe7]-bradykinin is is observed in the fragmentation of hyp3 bradykinin. The mass spectrometry. We observe that replacement of prolines Matrix-assisted laser desorption/ionization (MALDI) with methanol/acetonitrile and methanol/butanol systems on the methanol/acetonitrile. Butanol has a greater influence on methanol/butanol is between that of methanol/water and methanol/water system. The fragment ion abundance of post-source focusing technique. This work presented here primary study of solvent effect of methanol/water, sample preparation using acetonitrile relative to that in significantly less than that of bradykinin. A similar effect spectrometer, can be detected with the reflectron by using technique for the analysis of peptides and proteins. than when 1% TFA is present. the b-type ion formation. The abundance of internal fragment the fragment ion abundances of bradykinin when TFA is absent the sample preparation also increases fragment ion abundance. The total b-type and y-type fragment ions decreases for time-of-flight (TOF) mass spectrometry is a very powerful y-type cleavage significantly but have little influence on |
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| Item Description: | "Major subject: Chemistry". Vita. |
| Physical Description: | xi, 48 leaves : illustrations ; 28 cm. Also available online. Issued also on microfiche from Lange Micrographics. |
| Bibliography: | Includes bibliographical references: pages 45-47. |