Physiological effectors regulating gene expression of the hypertrehalosemic hormone in the cockroach, Blaberus discoidalis /

(Bld-HrTH) in the corpora cardiaca (CC) of Blaberus

Bibliographic Details
Main Author:	Lewis, Danielle K.
Format:	Thesis eBook
Language:	English
Published:	[Place of publication not identified] : [publisher not identified] ; 1996.
Subjects:	Major entomology.
Online Access:	Link to OAKTrust copy

Description
Summary:	(Bld-HrTH) in the corpora cardiaca (CC) of Blaberus 48 hours; but injections of glucose or trehalose, affected by starvation. The mechanical aspects of chewing analyses were used to elucidate the physiological factors and 4. Mating had no effect on Bld-HrTH MRNA content in and neural stimuli by stretch receptors of the foregut did Bld-HrTH message was determined to be approximately 0.5 kb. Bld-HrTH MRNA content declined by about 50% between days 0 carbohydrate-rich agar cubes given ad lib., and glucose carbohydrates passing through the gut do not appear to play a CC, ventral nerve cord, fat body, and brain were tested to content This baseline data was used to target the appropriate declined by approximately 75% by day 10, where it remained demonstrate that regulation of Bld-HrTH is influenced by age, determine if Bld-HrTH was produced solely in the CC or if discoidalis. Using northern blot hybridization analysis the Dot blot hybridization and northern blot hybridization female CC. Starvation for 10 days, but not dehydration, For both males and females, the highest content of Bld-HrTH gender and feeding Reproduction, water intake, the presence HrTH MRNA content in males, however, did not appear to be increased Bld-HrTH MRNA content 1.8-fold in females. Bld- influenced by glucose or trehalose. A biosynthetic model Initial studies using dot blot hybridization analysis focused MRNA was found immediately after the adult molt. Bld-HrTH MRNA was high in females between days 0 through 4 but not affect Bld-HrTH MRNA content. Feeding following of circulating carbohydrates within the hemocoel, and on the effects of age, gender, and sex on Bld-HrTH MRNA on the role of glucose and trehalose in Bld-HrTH regulation. presented in these studies, regulation of Bld-HrT'H in regulating transcript levels of the hypertrehalosemic hormone relating Bld-HrTH gene expression to trehalose utilization in relation to feeding is nutritionally based but is not significant role in Bld-HrTH MRNA content. Based on the data significantly affect Bld-HrTH MRNA content. These studies solutions given ad lib. in starved females did not stage for analyzing various aspects of feeding with emphasis starvation down-regulated Bld-HrTH transcripts by 30% after the cells has been proposed. there were other sites of synthesis. The CC was the only through day 30. In males, the trend was similar, but the tissue in which Bld-HrTH MRNA was detected. The size of the
Item Description:	"Major subject: Entomology". Vita.
Physical Description:	vii, 61 leaves : illustrations ; 28 cm. Also available online. Issued also on microfiche from Lange Micrographics.
Bibliography:	Includes bibliographical references: P. 51-60.