Mechanisms of AH receptor-mediated responses /
2 gene expression by estrogen in ER' breast cancer cell
| Main Author: | |
|---|---|
| Format: | Thesis eBook |
| Language: | English |
| Published: |
[Place of publication not identified] :
[publisher not identified] ;
1996.
|
| Subjects: | |
| Online Access: | Link to OAKTrust copy |
| Summary: | 2 gene expression by estrogen in ER' breast cancer cell Bcl-2 is a key regulator in cell apoptotic pathways, and bcl- bcl-2 niRNA levels were inhibited by TCDD in MCF-7 cells by 10 riM 2,3,7,8tetrachlorodibenzo-p-dioxin (TCDD) in Hepa CAT reporter gene. In cells cotranfected with the DRE-CAT Chloramphenicol acetyl transferase (CAT) activity was induced complex with c-fos and c-jun oncoprotein was investigated in construct and a c-fos expression plasmid, there was an containing a dioxin-responsive element (DRE) and a bacterial Cotransfection of Hepa 1 c I c7 cells with PRNH I I c Ah- CYPIAI gene and a c-fos expression plasmid also had minimal effect of Ah-responsiveness. Ah-and E2-responsiveness was estrogen-responsive VIT-TK-CAT plasmid, and only minimal expression were investigated in this study. The E2-induced G292, HOS(TE85), MG-63 and SAOS-2. The cells was transiently increase in basal CAT activity however Ah-responsiveness was induction of CAT activity were observed in some bone cells. inhibition on E2-induced CAT activity in T47D cells investigated in five human osteosarcoma cells namely U-2, lclc7 cells transfected with an Ah-responsive plasmid lines. The effects of TCDD on E2-mediated bcl-2 gene Nfinimal estrogen- and Ah-responsiveness was observed in no significant effect on basal or inducible CAT activity. not induced. Cells were also transiently transfected the nuclear Ah-receptor complex was detected in MG-63 cells. promoter subcloned PUCSVOCAT in T47D cells. responsive plasmid containing the 1142 to -2434 region of the The interaction of the nuclear aryl hydrocarbon (Ah) receptor these cell lines although relatively high levels of the transfected with Ah-responsive PRNH II c and CAT activity was transiently transfected with plasmids containing the bcl-2 unchanged. In contrast, cotransfection of Hepa lclc7 cells using RT-PCR method. Ten nM TCDD caused a significant wild-type Ah responsive mouse Hepa lclc7 cells. with the DRE-CAT construct and a c-jun expression plasmid had |
|---|---|
| Item Description: | "Major subject: Toxicology". Vita. |
| Physical Description: | ix, 79 leaves : illustrations ; 28 cm. Also available online. Issued also on microfiche from Lange Micrographics. |
| Bibliography: | Includes bibliographical references. |