Heat shock induced changes in enolase and HSP 70 in Echinochloa phyllopogon /

43'C (2h) and 28'C (1h). Enolase protein remained stable

Bibliographic Details
Main Author: Schweisguth, Allison Anne
Format: Thesis eBook
Language:English
Published: [Place of publication not identified] : [publisher not identified] ; 1996.
Subjects:
Online Access:Link to OAKTrust copy
Description
Summary:43'C (2h) and 28'C (1h). Enolase protein remained stable
43C (6h). Thermotolerance was inducible at a normally fatal
a manner sinfilar to that observed in other biological
anaerobic and heat stressed tissue as evidenced by protein
anaerobically germinated tissue, enolase was observed within
ASP enolase (EC 2.4.1.11, 2phospho-glycerate hydrolyase), a
be induced in response to general stress. Additionally,
both the cytoplasm and nucleus. In contrast to enolase
detected immunologically throughout the cell cytoplasm and
enolase in protecting E. phyllopogon from heat shock, but in
enolase were examined to determine the role of this protein
Exposure of plants to environmental insults results in the
Five day old E phyllopogon seedlings exhibited a very high
glycolytic enzyme, is induced at the protein, activity, and
heat tolerance; viability was maintained after exposure to
heat treatment (46'C, 6h) by pretreatment of seedlings at
heat treatment, either 43'C or 46'C for 6h. HSP70 was
HSP70, an HSP induced by heat shock in all organisms studied.
in anaerobically germinated or heat shocked tissue of E.
in enzyme activity. Synthesis of enolase was specifically
induced during the thermotolerance pretreatment where the
induction and its cytosolic and nuclear localization during
induction of distinct sets of proteins unique to that stress
maintenance under stress. The effects of heat shock on
nucleus in both control and heat shocked tissue. HSP70
organisms. Enolase may play a multifunctional role in
phyllopogon. Several proteins, including enolase, appear to
phyllopogon. The response of enolase was compared to that of
pretreatment, and its localization to the nucleus or cell
prolonged heat shock suggest it functions differently from
protein was detected proximal to the plasma membrane. In
proteins (HSPs). For example, during anaerobic stress, the
stability and accumulation during the thermotolerance
structural, as well as glycolytic, role in cellular
studies of various organisms indicate enolase may play a
such as anaerobic stress proteins (ASPS) or heat shock
synthesis induction, HSP70 synthesis was induced upon long
throughout all heat treatments without a significant increase
transcript levels in the flood tolerant species, Echinochloa
wall or plasma membrane under stress
Item Description:"Major subject: Plant Physiology".
In title, numerals are used.
Vita.
Physical Description:ix, 71 leaves : illustrations ; 28 cm.
Also available online.
Issued also on microfiche from Lange Micrographics.
Bibliography:Includes bibliographical references.